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Merck

H7140

Sigma-Aldrich

杂交溶液

for Northern and Southern blotting, liquid (2x concentrate)

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About This Item

分類程式碼代碼:
12161700
NACRES:
NA.25

品質等級

形狀

liquid (2x concentrate)

異物活動

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

儲存溫度

−20°C

一般說明

Sigma的杂交液是一种2倍即用型杂交缓冲液,适用于Northern和Southern印迹方案。

應用

杂交液已用于硝酸纤维素膜的杂交。它也已用于DNA杂交。
适合用作Northern和Southern印迹方案的杂交缓冲液。

成分

Sigma的杂交液含为10倍SSC和2倍Denhardt溶液中的200 μg/mL DNA:
200 μg/mL DNA
2mg牛血清白蛋白(BSA)
2mg聚蔗糖
2mg聚乙烯吡咯烷酮(PVP)
0.15 M柠檬酸钠
1.5 M NaCl

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Smooth muscle-specific expression of SV40 large TAg induces SMC proliferation causing adaptive arterial remodeling
Sindermann JR, et al.
American Journal of Physiology. Heart and Circulatory Physiology, 283(6), H2714-H2724 (2002)
Sambrook, J., et al.
Molecular Devices, 9-9 (1989)
Silicon nanonets for biological sensing applications with enhanced optical detection ability
Serre P, et al.
Biosensors And Bioelectronics, 68, 336-342 (2015)
Jürgen R Sindermann et al.
American journal of physiology. Heart and circulatory physiology, 283(6), H2714-H2724 (2002-10-22)
To study the effects of enhanced smooth muscle cell (SMC) proliferation on arterial vessel geometry in the absence of vessel trauma, we developed a transgenic mouse model expressing SV40 large T antigen under control of the 2.3-kb smooth muscle-myosin heavy
Joel W Slaton et al.
Molecular cancer research : MCR, 2(12), 712-721 (2005-01-07)
Protein serine/threonine kinase CK2 (formerly casein kinase 2) is a ubiquitous protein kinase that plays key roles in cell growth, proliferation, and survival. We have shown previously that its molecular down-regulation induces apoptosis in cancer cells in culture. Here, we

实验方案

Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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