The cyanogen bromide-activated agarose beads used to produce this product are 40-165 µm in diameter. Please see the link below to review additional information, including product preparation instructions:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/199/431/p4406dat.pdf
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生物来源
papaya (latex)
表单
lyophilized powder
比活
90-150 units/mL packed gel
分子量
21 kDa
标记范围
3,000-5,000 units per g agarose (at pH7.0 at 30 °C.)
基质
beaded agarose
储存温度
−20°C
相关类别
应用
Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies.[1] It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P4406 is agarose-linked papain and is provided as a lyophilized powder stabilized with lactose. Product P4406 has been used to digest purified anti-JMJD6 hamster monoclonal antibody into Fab fragments[2].
Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.
生化/生理作用
A cysteine protease that cleaves peptide bonds of basic amino acids, leucine or glycine. pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
Also hydrolyzes esters and amides.
Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
单位定义
One unit will hydrolyze 1.0 μmole of BAEE per min at pH 6.2 at 25°C, unless otherwise indicated below.
外形
Lyophilized powder, stabilized with lactose
警示用语：
Danger
危险声明
危险分类
Resp. Sens. 1 - Skin Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
其他客户在看
Yafei Liu et al.
Cell, 184(13), 3452-3466 (2021-06-18)
Antibodies against the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein prevent SARS-CoV-2 infection. However, the effects of antibodies against other spike protein domains are largely unknown. Here, we screened a series of anti-spike monoclonal antibodies from coronavirus disease 2019
Xia Hong et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(33), 14568-14572 (2010-08-04)
JMJD6 is a Jumonji C domain-containing hydroxylase. JMJD6 binds alpha-ketoglutarate and iron and has been characterized as either a histone arginine demethylase or U2AF65 lysyl hydroxylase. Here, we describe the structures of JMJD6 with and without alpha-ketoglutarate, which revealed a
Y. Ozari et al.
Journal of the Chemical Society. Chemical Communications, 295-295 (1974)
商品
木瓜蛋白酶是肽酶C1家族的一种半胱氨酸蛋白酶。它由单一多肽链组成，含有三个二硫键和一个酶活性所必需的巯基。
Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.
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Hello, could you please tell me the diameter of the agarose beads used in the product? I couldn't find the info on the store page or in the product description. (product number P4406-25UN) Best regards, Andria
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