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Merck

P3303

Sigma-Aldrich

内蛋白酶Asp-N 来源于莓实假单胞菌 突变菌株

suitable for protein sequencing, lyophilized powder

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1 VIAL
$433.00

$433.00


国内现货,预计发货时间2025年4月07日详情


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1 VIAL
$433.00

About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.56

$433.00


国内现货,预计发货时间2025年4月07日详情


获取大包装报价

等级

Proteomics Grade

质量水平

表单

lyophilized powder

分析物化学类别

amino acids

包装

vial of 2 μg

适用性

suitable for protein sequencing

储存温度

2-8°C

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一般描述

胞内蛋白酶 Asp-N 是一种金属内切蛋白酶。它来自于 莓实假单胞菌 的一个突变菌株,该菌株水解天冬氨酸和半胱氨酸残基 N 端的肽键。[1][2] Asp-N 因其对多肽的高度特异性剪切,在蛋白质组学中用于肽图分析和蛋白质序列工作。[3][4]

应用

来自莓实假单胞菌突变菌株的胞内蛋白酶 Asp-N 已用于特定蛋白质的消化,以制备肽并通过 MS(质谱)法分析生成的肽。[5][6][7]

象形图

Health hazardExclamation mark

警示用语:

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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S R Paik et al.
The Biochemical journal, 340 ( Pt 3), 821-828 (1999-06-09)
alpha-Synuclein is a component of the abnormal protein depositions in senile plaques and Lewy bodies of Alzheimer's disease (AD) and Parkinson's disease respectively. The protein was suggested to provide a possible nucleation centre for plaque formation in AD via selective
Sarah Murray et al.
Journal of virology, 80(12), 6171-6176 (2006-05-30)
Adeno-associated virus type 2 (AAV-2) capsid proteins have eight sequence motifs that are potential sites for O- or N-linked glycosylation. Three are in prominent surface locations, close to the sites of cellular receptor attachment and to neutralizing epitopes on or
T H Jensen et al.
The Journal of biological chemistry, 270(23), 13777-13784 (1995-06-09)
Human immunodeficiency virus encodes the regulatory protein Rev, which is required for expression of viral structural proteins. It binds to an RNA element (RRE) in the viral transcript and up-regulates the cytoplasmic appearance of unspliced and singly spliced viral mRNA.
Wilson KJ, et al.
Methods in Protein Sequence Analysis: Proceedings of the 7th International Conference, Berlin, July 3?8, 1988, 310-310 (1988)
Takatoshi Ohkuri et al.
Journal of biochemistry, 154(4), 333-340 (2013-07-16)
A method was previously established for evaluating Asn deamidation by matrix-assisted laser desorption/ionization time of flight-mass spectrometry using endoproteinase Asp-N. In this study, we demonstrated that this method could be applied to the identification of the deamidation site of the

实验方案

An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).

基于优化的 LC-MS/MS 工作流程,通过过滤辅助样品制备 (FASP) 对阿达木单抗 (Humira) 绘制低伪影胰蛋白酶酶解和肽图谱。

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