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P1585

Sigma-Aldrich

PMA

for use in molecular biology applications, ≥99% (HPLC)

别名:

佛波醇12-十四酸酯13-乙酸酯, NF-κB活化剂, PKC活化剂, 佛波醇酯, 巴豆油, 蛋白激酶C活化剂, 12-O-十四烷酰基佛波醇13-乙酸酯, 4β,9α,12β,13α,20-五羟基噻吩-1,6-二烯-3-酮12-十四烷酸酯13-乙酸酯, PMA, TPA

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1 MG
$199.00
5 MG
$475.00
10 MG
$1,210.00
25 MG
$1,750.00

$199.00

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1 MG
$199.00
5 MG
$475.00
10 MG
$1,210.00
25 MG
$1,750.00

About This Item

经验公式（希尔记法）:
C36H56O8
CAS号:
16561-29-8
分子量:
616.83
Beilstein:
2407201
MDL號碼:
MFCD00036736
分類程式碼代碼:
12352200
PubChem物質ID:
24898219
NACRES:
NA.52

$199.00

请联系客服了解存货情况
获取大包装报价

等級

for molecular biology

品質等級

200

化驗

≥99% (HPLC)

形狀

(Lyophilisate)

分子量

616.83  g/mol

儲存條件

protect from light

溶解度

DMSO: soluble (DMSO solutions can be stored dark at −20 °C for at least six months.)
ethanol: soluble (Dissolve in water-miscible organic solvent prior to preparing aqueous solutions)

儲存溫度

−20°C

SMILES 字串

CCCCCCCCCCCCCC(=O)O[C@@H]1[C@@H](C)[C@@]2(O)C(C=C(CO)C[C@@]3(O)C2C=C(C)C3=O)C4C(C)(C)[C@]14OC(C)=O

InChI

1S/C36H56O8/c1-7-8-9-10-11-12-13-14-15-16-17-18-29(39)43-32-24(3)35(42)27(30-33(5,6)36(30,32)44-25(4)38)20-26(22-37)21-34(41)28(35)19-23(2)31(34)40/h19-20,24,27-28,30,32,37,41-42H,7-18,21-22H2,1-6H3/t24-,27+,28-,30-,32-,34-,35-,36-/m1/s1

InChI 密鑰

PHEDXBVPIONUQT-RGYGYFBISA-N

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一般說明

PMA（佛波醇12-十四酸酯13-乙酸酯）是一种蛋白激酶C（PKC）的激活剂和已知的肿瘤促进剂。PMA还表现出致裂（染色体断裂）活性。[1]
佛波醇12-肉豆蔻酸酯13-乙酸酯可诱导PLD1（磷脂酶D1）活化，但不会诱导PLD2（磷脂酶D2）活化。[2]

PMA通过蛋白激酶C (PKC)信号传导途径，充当以下机制相关的肿瘤启动子：

  • 基因转录
  • 细胞生长
  • 细胞分化
  • 细胞程序性死亡
  • 免疫途径
  • 受体脱敏

佛波醇12-肉豆蔻酸酯13-乙酸酯用于研究：

  • 生长因子
  • 激素
  • 细胞因子介导的细胞生长和分化调控

應用

PMA已用于：
  • 胞内细胞分裂素检测[3]
  • CellROX氧化应激分析[4]
  • Huh7细胞刺激，以研究二肽基肽酶9 (DPP9)的重新分布[5]
  • 用作慢性淋巴细胞性白血病(CLL)细胞B细胞受体(BCR)刺激的阳性对照[6]
  • 单核细胞(THP -1)向巨噬细胞的分化[7]

适用于
  • 作为标准品用于分子生物学研究
  • 肿瘤启动子分析研究
  • 促进细胞生长

生化／生理作用

有效的肿瘤启动子；体内和体外活化蛋白激酶C (PKC)。

特點和優勢

  • 刺激Jurkat细胞产生白介素2
  • 刺激T细胞
  • 蛋白激酶C (PKC)活化剂
  • NF-κB活化剂
  • 促细胞生长
  • 用于肿瘤启动子分析研究
  • 分子生物学级

注意

光敏感的，避光保存。

分析報告

  • 刺激Jurkat细胞产生白介素2
  • 刺激T细胞
  • 蛋白激酶C (PKC)活化剂
  • NF-κB活化剂
  • 促细胞生长
  • 用于肿瘤启动子分析研究
  • 分子生物学级

訊號詞

Danger

危險分類

Acute Tox. 1 Dermal - Acute Tox. 1 Inhalation - Acute Tox. 2 Oral - Carc. 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類（WGK）

WGK 3

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000412861
0000412861
0000402755
0000395055
0000392913

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二氢罗丹明123 ≥95%

Sigma-Aldrich

D1054

二氢罗丹明123

Identification of a potential biomarker for FABP4 inhibition: the power of lipidomics in preclinical drug testing
Suhre K, et al.
Journal of Biomolecular Screening, 16(5), 467-475 (2011)
Biological properties and measurement of phospholipase D activation by ADP-ribosylation factor (ARF)
Cockcroft S, et al.
Methods in Enzymology, 329, 355-372 (2001)
Proteomic analysis of B-cell receptor signaling in chronic lymphocytic leukaemia reveals a possible role for kininogen
Kashuba E, et al.
Journal of Proteomics, 91(2), 478-485 (2013)
Tumour promoter phorbol-12-myristate-13-acetate induces chromosomal damage via indirect action.
I Emerit et al.
Nature, 293(5828), 144-146 (1981-09-10)
The immune responses of central and effector memory BCG-specific CD4+ T cells in BCG-vaccinated PPD+ donors were modulated by Treg cells
Li L, et al.
Immunobiology, 216(4), 477-484 (2011)
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相关内容

Phorbol 12-myristate 13-acetate Molecular Biology Reagent Protocol

T-cell activation is normally triggered by the interaction of a cell surface receptor to its specific ligand molecule

Questions

1–10 of 10 Questions  

  1. How can I resuspend properly the PMA into the shipped vial? Can I open the vial?

    1 answer

    1. The packaging consists of an amber serum vial sealed with a septum and an aluminum crimp cap. The product is typically dissolved in the vial by injecting the appropriate volume of the selected solvent through the septum using a syringe. Additional solubility and solution stability information can be reviewed in the datasheet available for the general use product, P8139. See the link below to review these recommendations:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/816/596/p8139pis.pdf

      Helpful?

  2. What is the stock concentration of the PMA when adding DMSO. and How much of DMSO do we add?

    1 answer

    1. Suitability Assay - 2.5 ml of Jurkat cells (1 x 10e6 cells/mL) and 2.5 mLof fresh media (RPMI 1640 + 10% fetal calf serum containing 10 ml/l penicillin-streptomycin) were added to 25 cm2 culture bottles.
      The following additions were made in duplicate.
      a. Control - no additions
      b. 1 µg/ml PHA Control - add 10 µl PHA stock solution (0.5 mg/ml PHA in filter-sterilized PBS)
      c. 1 µg/ml PHA + 50 ng/ml PMA – 10 µl PHA stock solution + 2.5 µl PMA stock solution (100 µg/ml PMA in DMSO).

      As stated in part c. - the PMA stock solution is 100µg/ml PMA in DMSO.

      Please see the datasheet for more details:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/311/794/p1585dat.pdf

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. What is the difference between Phorbol 12-myristate 13-acetate, Product P8139 and molecular biology grade Product P1585?

    1 answer

    1. Product P1585 has been tested and found suitable for stimulation of interleukin 2 production of Jurkat cells. Product P8139 has not tested in this application.

      Helpful?

  5. There isn't any powder in the Phorbol 12-myristate 13-acetate, Product P1585, vial?

    1 answer

    1. The product is usually a clear film on the walls of the vial and may not be easily visible.

      Helpful?

  6. What is the stability of stock solutions of Phorbol 12-myristate 13-acetate, Product P1585, and how should I store them?

    1 answer

    1. Stock solutions in DMSO can be stored in the dark as aliquots at -20°C for at least six months.

      Helpful?

  7. How can I dissolve Phorbol 12-myristate 13-acetate, Product P1585?

    1 answer

    1. Phorbol 12-myristate 13-acetate (PMA) is soluble in DMSO at a 20 mM concentration.  It is also soluble in ethanol.

      Helpful?

  8. What is the storage temperature for Phorbol 12-myristate 13-acetate, Product P1585?

    1 answer

    1. Phorbol 12-myristate 13-acetate should be stored at -20°C.

      Helpful?

  9. How much Phorbol 12-myristate 13-acetate, Product P1585, product should I use?

    1 answer

    1. A concentration of 50 ng/ml was used on Jurkat cells.

      Helpful?

  10. How is Product P1585, Phorbol-12-myristate-13-acetate, packaged?

    1 answer

    1. P1585-1MG, P1585-10MG and P1585-25MG come to us pre-packaged by our supplier; all three sizes are packaged the same way. The packaging consists of an amber serum vial with a septum and an aluminum crimp cap. The septum has a PTFE (Teflon) liner, so the material inside the vial could only come in contact with glass and Teflon.

      Helpful?

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