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MAK142

Sigma-Aldrich

荧光测定细胞内活性氧试剂盒

sufficient for 200 fluorometric tests (Deep red)

别名:

细胞内ROS荧光检测试剂盒

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1 KIT
$476.00

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1 KIT
$476.00

About This Item

EC 号:
200-664-3
UNSPSC代码:
12161503
NACRES:
NA.25

$476.00

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获取大包装报价

用途

sufficient for 200 fluorometric tests (Deep red)

应用

cosmetics
food and beverages

检测方法

fluorometric

相关疾病

neurological disorders; cancer

储存温度

−20°C

相关类别

一般描述

由于在有氧呼吸期间氧气的减少以及细胞内的各种酶系统产生活性氧物质（ROS）。在生理水平，ROS 有助于细胞信号传导和宿主防御。[1]增加的 ROS 产生，高于生物系统的解毒能力，导致氧化应激和细胞损伤。[2]细胞的主要损害是由 ROS 诱导的大分子改变，如膜脂、必需蛋白和 DNA 中的多不饱和脂肪酸。ROS 已经与疾病状态有关，例如阿尔茨海默′病、帕金森′病、癌症和衰老。[3]

应用


  • 漂白粉二乙酯在LPS刺激的RAW264.7细胞中通过TLR4/MyD88信号通路显示抗炎和抗氧化活性。：这篇文章利用荧光测定法探讨了漂白粉二乙酯的抗炎和抗氧化特性，证明ROS检测在天然化合物治疗作用分子机制中的作用（Yang S et al., 2019）。

适用性

本试剂盒适于通过酶标仪、流式细胞仪或带TRITC滤镜的荧光显微镜检测细胞内的ROS。

原理

荧光细胞内 ROS 试剂盒提供灵敏的一步荧光检测法，用于在孵育 1 小时后检测活细胞中的细胞内 ROS（特别是超氧化物和羟基自由基）。ROS 与定位于细胞质的荧光传感器反应，产生与存在的 ROS 量成比例的荧光产物。该测定可以 96 或 384 多孔板形式进行，并且在 λex = 650/λem = 675 nm 处进行检测。

储存分类代码

10 - Combustible liquids

WGK

WGK 3

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
3510351
3510351
3181881
3181881
2781712

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  1. How is shipping temperature determined? And how is it related to the product storage temperature?

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    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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