While the specifications for this probe do not include assaying for specific applications, there are many publications showing its use in flow cytometry applications:
https://www.sciencedirect.com/science/article/pii/S0022030215000909
https://iubmb.onlinelibrary.wiley.com/doi/pdf/10.1002/iub.1895
https://www.tandfonline.com/doi/pdf/10.1080/21655979.2021.1994737
https://journal.waocp.org/article_91422_6ccd2a19eb9159073edeb8f9ce0be660.pdf
https://pmc.ncbi.nlm.nih.gov/articles/PMC7842666/
推荐产品
产品名称
2',7'-二氯荧光素二乙酸酯, ≥97%
品質等級
化驗
≥97%
形狀
powder
儲存溫度
−20°C
SMILES 字串
CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C(c2cc1Cl)c4ccccc4C(O)=O
InChI
1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
InChI 密鑰
PXEZTIWVRVSYOK-UHFFFAOYSA-N
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一般說明
2′,7′-二氯二氢荧光素二乙酸酯（DCFH-DA）是一种对氧化敏感的荧光染料，用于检测细胞内活性氧（ROS）水平。DCFH-DA是一种细胞渗透性非荧光探针，可被细胞内酯酶水解为DCFH，当被细胞内的ROS氧化时形成荧光DCF。[1]
The excitation wavelength is 504 nm and emission wavelength is 529 nm in ethanol. The use of DMSO as the solvent has not been determined, however various publications suggest that it is suitable.
應用
适用于：敏感并快速定量活性氧，以响应氧化代谢；微孔板法，以检测吞噬细胞中的氧化产物；髓样分化定量多孔测定。
儲存和穩定性
本品应在-20℃下以粉末形式保存。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
個人防護裝備
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Yeonhwa Song et al.
Journal of experimental & clinical cancer research : CR, 36(1), 38-38 (2017-03-04)
CD133-positive cells in hepatocellular carcinoma (HCC) exhibit cancer stem cell (CSC)-like properties as well as resistance to chemotherapeutic agents and ionizing radiation; however, their function remains unknown. In this paper, we identified a hitherto unknown mechanism to overcome CD133-induced resistance
Pornthip Chaichompoo et al.
Journal of clinical pathology, 72(8), 520-524 (2019-04-24)
Iron overload is a major factor contributing to the overall pathology of thalassaemia, which is primarily mediated by ineffective erythropoiesis and shorter mature red blood cell (RBC) survival. Iron accumulation in RBCs generates reactive oxygen species (ROS) that cause cellular
Yuan Ji et al.
Journal of agricultural and food chemistry, 67(32), 9060-9069 (2019-07-25)
Glutathione S-transferases (GSTs) play an active role in the development of drug resistance by numerous cancer cells, including melanoma cells, which is a major cause of chemotherapy failure. As part of our continuous effort to explore why dietary polyphenols bearing
Fatemeh Eini et al.
Molecular reproduction and development, 86(8), 1053-1066 (2019-06-19)
Although in-vitro maturation (IVM) of oocytes has been presented as an alternative treatment to traditional stimulated in-vitro fertilization, the culture condition can be improved by natural antioxidants. Thus, we investigated the protective effect of Thymoquinone (TQ) during IVM in the
Esha Madan et al.
Nature, 572(7768), 260-264 (2019-07-26)
In humans, the adaptive immune system uses the exchange of information between cells to detect and eliminate foreign or damaged cells; however, the removal of unwanted cells does not always require an adaptive immune system1,2. For example, cell selection in
商品
氧化应激，在一部分上，是由多种细胞过程产生的活性氧介导，并由诸如酶清除剂或抗氧化剂调节剂等细胞抗氧化剂机制所控制的。自由基，如活性氧，会通过细胞引起细胞损伤。
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
DISCOVER Bioactive Small Molecules for Nitric Oxide & Cell Stress Research
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Is D6883 2´,7´-Dichlorofluorescin diacetate suitable for flow cytometry analyses?
1 answer-
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What is the protocol for measuring ROS using D6883? Specifically, what kind of media or buffer is used with this product?
1 answer-
The specific protocol for measuring ROS using D6883 and the type of media or buffer to be used with this product are not available. However, typically, this product is added to a buffer solution shortly before assessing the fluorescent signal. For further guidance, refer to the following citations for relevant information:
1. Novel Antioxidant Tripeptide "ACQ" Can Prevent UV-Induced Cell Death and Preserve the Number of Epidermal Stem Cells
PMID: 261996772. Detection of reactive oxygen species (ROS) by the oxidant-sensing probe 2',7'-dichlorodihydrofluorescein diacetate in the cyanobacterium Anabaena variabilis PCC 7937
PMID: 2057064These publications may provide insights into the protocol for using D6883 and the type of media or buffer used with this product.
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What concentration of DCF can I use for A549 cells?
1 answer-
This product has not been tested for use in cell culture. A search of the literature suggests working concentrations range from 5 µM to 0.1 mM. See the link below to a publication that specifically looked at A549 cells using several dyes, including DCFDA:
https://pubs.rsc.org/en/content/articlehtml/2017/ra/c7ra01025gHelpful?
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What concentration of DCF can I use for THP-1 monocyte cell line?
1 answer-
The optimal concentration of 2′,7′-Dichlorofluorescin diacetate (DCFDA) for use with THP-1 monocytes will need to be determined by the end-user. See the link below to a paper that reported using DCF at 40 uM with THP-1 cells.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961584/Helpful?
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Is it possible to view this probe under a fluorescence microscope if the probe is solubilised in DMSO? What are the excitation and absorption wavelengths?
1 answer-
The excitation wavelength is 504 nm and the emission wavelength is 529 nm in ethanol. The use of DMSO as the solvent has not been determined, however, various publications suggest that it is suitable. See the link below for an example:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712457/Helpful?
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Protocol please! Thank you very much!
1 answer-
It may be helpful to view the following articles as there is not a specific protocol for this product:
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Can the product be stored in powder form and if its possible what is the storage temp?
1 answer-
This product should be stored in powdered format at -20°C.
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What is the solution stability of Product D6883, 2´,7´-Dichlorofluorescin diacetate?
1 answer-
This product can be stored at -20°C for up to six months in solvents such as DMSO, ethanol, or DMF (N,N-dimethylformamide).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the excitation and emission wavelength for Product D6883, 2´,7´-Dichlorofluorescin diacetate?
1 answer-
The excitation wavelength is 504 nm and emission wavelength is 529 nm (in ethanol).
Helpful?
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