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35845

Sigma-Aldrich

2′,7′-二氯荧光素二乙酸酯

BioReagent, suitable for fluorescence, ≥95% (HPLC)

别名:

DCFHDA, 双氯荧光黄乙酸乙酯

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5 G
$1,120.00

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5 G
$1,120.00

About This Item

经验公式（希尔记法）:
C24H14Cl2O7
CAS号:
2044-85-1
分子量:
485.27
Beilstein:
68488
MDL號碼:
MFCD00037501
分類程式碼代碼:
12352108
PubChem物質ID:
57649438
NACRES:
NA.32

$1,120.00

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產品線

BioReagent

品質等級

100

化驗

≥95% (HPLC)

mp

232-234 °C (lit.)

溶解度

DMSO: soluble

螢光

λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)

適合性

suitable for fluorescence

儲存溫度

2-8°C

SMILES 字串

CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3

InChI 密鑰

VQVUBYASAICPFU-UHFFFAOYSA-N

相关类别

一般說明

2′,7′-二氯荧光素二乙酸酯，也称为 DCFHDA 或 Dcfh-DA，是一种敏感的细胞可渗透氧化还原探针。亲脂性非荧光 Dcfh-DA 通过被动扩散和脱乙酰作用很容易穿过细胞膜。脱酰产物是一种对氧化剂敏感的 2′,7′-二氯荧光素（DCHF）。 DCHF 随后被氧化以形成高荧光 DCF，这是被测量的。[1]

應用

2′,7′-二氯荧光素二乙酸酯主要用于检测活性氧（ROS）中间体并量化细胞中的过度 氧化应激 。[1]

Dcfh-DA 用于定量测定，以测量纳米颗粒处理的细胞中的氧化应激。[2]

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCL7891
BCCM2610
BCCL2220
BCCK3188
BCCJ3620

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Michal Afri et al.
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2',7'-Dichlorodihydrofluorescein diacetate (DCFH-DA) is commonly used to detect the generation of reactive oxygen intermediates and for assessing the overall oxidative stress in toxicological phenomenon. It has been suggested that DCFH-DA crosses the cell membrane, subsequently undergoing deacetylation by intracellular esterases.
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Questions

1–8 of 8 Questions  

  1. Can I get the protocol? Thank you

    1 answer

    1. A protocol is not available for this compound.

      Please see the link below to review a publication that may be helpful.
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712457/

      Helpful?

  2. What is the shelf life of this product, as a powder, and once dissolved?

    1 answer

    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Stock solutions of this item in organic solvents are stable for at least 6 months if stored at -20°C.

      Helpful?

  3. What are the optical properties of the hydrolysis product of 2´,7´-Dichlorofluorescein diacetate, Product 35845?

    1 answer

    1. Excitation maximum is at 504 nm; emission maximum is at 529 nm (in 0.1 M Tris pH 8.0 following treatment with an esterase).

      Helpful?

  4. What are the recommended storage conditions for 2´\,7´-Dichlorofluorescein diacetate, Product 35845?

    1 answer

    1. Product no. 35845 should be stored in a refrigerator or coldroom at 2-8°C.

      Helpful?

  5. Can Product 35845, 2´,7´-Dichlorofluorescein diacetate be used for ROS (Reactive Oxygen Species) detection?

    1 answer

    1. Yes, Product 35845, 2´,7´-Dichlorofluorescein diacetate can be used for ROS detection by FACS analysis.

      Helpful?

  6. In what solvents is 2´,7´-´Dichlorofluorescein diacetate, Product 35845, soluble?

    1 answer

    1. It is soluble in DMF, ethanol (2 mg/mL), DMSO (10 mg/mL).

      Helpful?

  7. What is required to get 2´,7´-Dichlorofluorescein diacetate, Product 35845, to fluoresce?

    1 answer

    1. 2',7'-dichlorofluorescein diacetate fluoresces only after cleavage of the acetyl groups.

      Helpful?

  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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