Recombinant Molecular Weight Marker has been used for determining the size of protein bands in western blot and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).[1][2]
其他说明
The recombinant molecular weight standard for SDS-PAGE contains seven precisely sized proteins, M.W. 15, 25, 35, 50, 75, 100 and 150 kDa. Recombinant proteins produce sharp bands for precise size determination since they are not glycosylated.
外形
Solution containing approx. 0.8 mg/ml total protein in 125 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 200 mM 2-mercaptoethanol, 0.007% bromphenol blue.
Cloning, expression and functional characterisation of chitinase from larvae of tomato moth (Lacanobia oleracea): a demonstration of the insecticidal activity of insect chitinase.
Fitches E, et al.
Insect Biochemistry and Molecular Biology, 34, 1037-1050 (2004)
Cell cycle dependent telomere regulation by telomerase in human bone marrow mesenchymal stem cells.
Zhao YM, et al.
Biochemical and Biophysical Research Communications, 369, 1114-1119 (2008)
Clinical and vaccine immunology : CVI, 15(8), 1292-1300 (2008-06-27)
Earlier studies have demonstrated in A/Sn mice highly susceptible to Chagas' disease protective immunity against lethal Trypanosoma cruzi infection elicited by vaccination with an open reading frame (ORF) expressed by amastigotes. In our experiments, we used this mouse model to
