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品質等級
200
400
形狀
lyophilized powder
用途
sufficient for 10-20 large gels (16 × 18 cm)
sufficient for 20-30 mini-gels (10 × 10 cm)
vial sufficient for 50-100 PhastGels
分子量
6,500-66,000 Da
儲存溫度
2-8°C
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相关类别
應用
SigmaMarkers设计用于Laemmli SDS-PAGE、PhastSystem (Pharmacia) 、以及Schagger和von Jagow 的SDS-PAGE(三甲基甘氨酸)。 SigmaMarkers涵盖大多数蛋白质或其亚基共有的分子量范围。宽、高和低范围分子量标准品与样品缓冲液一起冻干,便于用去离子水溶解后即可使用。该分子量标准品已经过配制,可在电泳和考马斯蓝染色后产生大致相等强度的明确条带分布。
其他說明
Protein (mol. wt.)
Aprotinin, bovine lung (6,500)
α-Lactalbumin, bovine milk (14,200)
Trypsin inhibitor, soybean (20,000)
Trypsinogen, bovine pancreas (24,000)
Carbonic anhydrase, bovine erythrocytes (29,000)
Glyceraldehyde-3-phosphate dehydrogenase, rabbit muscle (36,000)
Ovalbumin, chicken egg (45,000)
Albumin, bovine serum (66,000)
Aprotinin, bovine lung (6,500)
α-Lactalbumin, bovine milk (14,200)
Trypsin inhibitor, soybean (20,000)
Trypsinogen, bovine pancreas (24,000)
Carbonic anhydrase, bovine erythrocytes (29,000)
Glyceraldehyde-3-phosphate dehydrogenase, rabbit muscle (36,000)
Ovalbumin, chicken egg (45,000)
Albumin, bovine serum (66,000)
Will yield eight protein bands with Coomassie Blue staining.
重構
Reconstitution with 100 μl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 10 mM TCEP, 2% SDS, and 0.01% bromophenol blue.
法律資訊
SigmaMarker is a trademark of Sigma-Aldrich Co. LLC
訊號詞
Danger
危險分類
Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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