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Merck

L7002

Sigma-Aldrich

L-Lysine p-nitroanilide dihydrobromide

≥98% (TLC), suitable for ligand binding assays

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250 MG
$69.40
1 G
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5 G
$669.00

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250 MG
$69.40
1 G
$139.00
5 G
$669.00

About This Item

经验公式(希尔记法):
C12H18N4O3 · 2HBr
CAS号:
分子量:
428.12
MDL编号:
UNSPSC代码:
12352209
PubChem化学物质编号:
NACRES:
NA.26

$69.40


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产品名称

L-Lysine p-nitroanilide dihydrobromide,

方案

≥98% (TLC)

表单

powder

技术

ligand binding assay: suitable

颜色

white to off-white

储存温度

2-8°C

SMILES字符串

Br.NCCCC[C@H](N)C(=O)Nc1ccc(cc1)N(=O)=O

InChI

1S/C12H18N4O3.BrH/c13-8-2-1-3-11(14)12(17)15-9-4-6-10(7-5-9)16(18)19;/h4-7,11H,1-3,8,13-14H2,(H,15,17);1H/t11-;/m0./s1

InChI key

FJNCJWJSSUJZMS-MERQFXBCSA-N

应用

L-Lysine p-nitroanilide may be used as a substrate to study the specificity and kinetics of lysine aminopeptidase(s) and various proteinases.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

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Akifumi Kawamura et al.
Biomacromolecules, 6(2), 627-631 (2005-03-15)
The amidase activity of bovine pancreas trypsin in water-soluble complexes with poly(ethylene glycol)-block-poly(alpha,beta-aspartic acid) (PEG-PAA) was evaluated by a colorimetric assay using L-lysine p-nitroanilide as a substrate. The enzymatic reaction of trypsin was accelerated through the complexation with PEG-PAA. By
Bernardo Ramírez-Zavala et al.
FEMS microbiology letters, 235(2), 369-375 (2004-06-09)
A lysine aminopeptidase was purified from the yeast Kluyveromyces marxianus. This enzyme was purified 100-fold from a soluble extract obtained at 100,000g. The purification procedure consisted in fractionated precipitation with ammonium sulfate and five chromatography steps. The native enzyme had
Natasa Bozić et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 134(2), 231-241 (2003-02-06)
Exopeptidases of Morimus funereus larvae were partially purified and characterized. Specific leucyl aminopeptidase (LAP) activity was increased eight-fold by gel filtration of the crude midgut extract. The partially purified LAP had a molecular mass greater than 100 kDa with pH
Priscilla L Phillips et al.
PloS one, 13(11), e0207295-e0207295 (2018-11-13)
The oral obligate anaerobe Porphyromonas gingivalis possesses a small conserved transcript PG_RS02100 of unknown function we previously identified using small RNA-seq analysis as expressed during logarithmic growth. In this study, we sought to determine if PG_RS02100 plays a role in

Questions

  1. Can we use L-Lysine p-nitroanilide dihydrobromide as a substrate for enzyme assay of lysine gingipain protease?

    1 answer
    1. Unfortunately, no data indicating whether L7002 L-Lysine p-nitroanilide dihydrobromide might be a suitable substrate for the enzyme is collected. A search of the BRENDA enzyme database mentioned N-p-tosyl-Gly-Pro-Lys-4-nitroanilide but provided no evidence that L-Lysine p-nitroanilide is a substrate.

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