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Merck
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B4500

Sigma-Aldrich

Nα-苯甲酰基-L-精氨酸乙酯 盐酸盐

trypsin substrate, chromogenic, ≥97% (HPLC), powder

别名:

BAEE

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About This Item

经验公式（希尔记法）:
C15H22N4O3 · HCl
CAS号:
2645-08-1
分子量:
342.82
Beilstein:
3781694
EC號碼:
220-157-0
MDL號碼:
MFCD00012579
分類程式碼代碼:
12352204
PubChem物質ID:
24891777
NACRES:
NA.32

product name

Nα-苯甲酰基-L-精氨酸乙酯 盐酸盐, trypsin substrate

化驗

≥97% (HPLC)

形狀

powder

溶解度

water: 50 mg/mL, clear, colorless

儲存溫度

2-8°C

SMILES 字串

Cl[H].CCOC(=O)[C@H](CCCNC(N)=N)NC(=O)c1ccccc1

InChI

1S/C15H22N4O3.ClH/c1-2-22-14(21)12(9-6-10-18-15(16)17)19-13(20)11-7-4-3-5-8-11;/h3-5,7-8,12H,2,6,9-10H2,1H3,(H,19,20)(H4,16,17,18);1H/t12-;/m0./s1

InChI 密鑰

HIXDELXKSSLIKB-YDALLXLXSA-N

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一般說明

Nα-苯甲酰基-L-精氨酸乙酯盐酸盐是胰蛋白酶底物。是用于光谱测定的非蛋白质底物。

應用

  • N

  • α-苯甲酰基-L-精氨酸乙酯盐酸盐作为底物，用于：
  • 在牙龈卟啉单胞菌（ P. gingivalis ）囊泡中测定肽基精氨酸脱亚氨酶（PPAD）
  • 测定番木瓜植物部位酶提取物的蛋白水解活性
  • 测定大鼠尾肌腱束的胰蛋白酶活性

基底

胰蛋白酶的原型底物。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

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Chensong Pan et al.
Analytical and bioanalytical chemistry, 387(1), 193-204 (2006-11-07)
Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) is widely used in a variety of fields because it has the characteristics of speed, ease of use, high sensitivity, and wide detectable mass range for obtaining molecular weights and for structural characterization of
Ying-Qing Yu et al.
Analytical chemistry, 75(21), 6023-6028 (2003-11-01)
Improved in-solution tryptic digestion of proteins in terms of speed and peptide coverage was achieved with the aid of a novel acid-labile anionic surfactant (ALS). Unlike SDS, ALS solubilizes proteins without inhibiting trypsin or other common endopeptidases activity. Trypsin activity
Kinetic and Structural Differentiation of Trypsin from Different Origins
Aithal M, et al.
BioPharm International, 30(1), 38-+-38-+ (2017)
Mohammad K Hossain et al.
Colloids and surfaces. B, Biointerfaces, 181, 85-93 (2019-05-28)
Electric current responsive magnetic composite particles are prepared in three steps. In the first step, spherical and mesoporous submicrometer-sized magnetic iron oxide (Fe3O4) core particles are prepared by solvothermal method. Then magnetic Fe3O4 particles are functionalized with amine groups using
Optima of Trypsin-Catalyzed Hydrolysis and Its Inhibition Determined by SDS-PAGE
Zhou X, et al.
Advances in Enzyme Research, 4(01), 1-1 (2016)
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实验方案

Enzymatic Assay of Trypsin Inhibitor

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胰蛋白酶（EC 3.4.21.4）的酶学测定方案

本方案适用于使用Nα-苯甲酰基-L-精氨酸乙酯（BAEE）作为底物测定胰蛋白酶活性。本方案是基于连续分光光度速率测定（A253，光程= 1cm）方案：

Trypsin Assay Procedure (EC 3.4.21.4)

Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.

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