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Merck

L0881

Sigma-Aldrich

花生的凝集素

lyophilized powder, Affinity-purified

别名:

PNA, 花生凝集素

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5 MG
$180.00
10 MG
$331.00
25 MG
$687.00

About This Item

UNSPSC代码:
12352202
NACRES:
NA.32

$180.00


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表单

lyophilized powder

质量水平

效能

<0.1 μg per mL agglutination activity

质量

Affinity-purified

杂质

salt, free

储存温度

−20°C

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一般描述

Arachis hypogaea (花生)中提取的凝集素是分子量 110 kDa的同四聚体蛋白。 [1]

应用

Arachis hypogaea (花生)中提取的凝集素用于:
  • 检测 Thomson-Friedenreich 抗原[2]
  • 作为PBS的成分,研究其对细胞活力的影响[3]
  • 研究其阻断结合位点的能力 [4]

生化/生理作用

Arachis hypogaea (花生)中提取的凝集素对 Thomson − Friedenreich 抗原的 β -D-Gal(1-3)-D-galNAc 残基具有特异性。 [5][1] 在许多鳞状细胞癌诊断和预后中,是最广泛使用的标志物。[5]
花生凝集素不会凝集正常的人类红细胞,但会强烈凝集神经氨酸酶处理的红细胞。花生凝集素具有与人血清中的抗 T 抗体相似的有效抗 T 活性。凝集素可用于区分人淋巴细胞亚群。

分析说明

凝集活性以 μg/ml 表示,并根据 1 mg/mL 溶液在磷酸盐缓冲盐水 (pH 6.8) 中的系列稀释液测定。该活性是在 25°C 下孵育 1 h 后凝集 2% 神经氨酸酶处理的人 O 型红细胞悬液的最低浓度。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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In vivo tumor cell adhesion in the pulmonary microvasculature is exclusively mediated by tumor cell-endothelial cell interaction
Gassmann P
BMC Cancer, 10(1), 177-177 (2010)
Expression of peanut agglutinin-binding mucin-type glycoprotein in human esophageal squamous cell carcinoma as a marker
Kannan S, et al.
Molecular Cancer, 2(1), 38-38 (2003)
Alejandro J Cagnoni et al.
Acta crystallographica. Section D, Structural biology, 76(Pt 11), 1080-1091 (2020-11-03)
Carbohydrate-lectin interactions are involved in important cellular recognition processes, including viral and bacterial infections, inflammation and tumor metastasis. Hence, structural studies of lectin-synthetic glycan complexes are essential for understanding lectin-recognition processes and for the further design of promising chemotherapeutics that
Adhesion of plant roots to poly-L-lysine coated polypropylene substrates
Towler MJ and Weathers PJ
Journal of Biotechnology, 101(2), 147-155 (2003)
Bridgette T Peal et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 38(8), 1826-1835 (2020-01-23)
The objectives of this study were to evaluate temporal changes in lubricin, hyaluronan (HA), and HA molecular weight (MW) distributions in three distinct models of equine joint injury affecting the carpal (wrist), tarsal (ankle), and femoropatellar (knee) joints. To establish

Questions

1–3 of 3 Questions  
  1. Is the peanut lectin (Lectin from Arachis hypogaea) a glycoprotein?  If so, what is the carbohydrate content?

    1 answer
    1. Peanut lectin is not a glycoprotein. Although peanut lectin binds specifically to a particular carbohydrate (beta-gal(1-->3)galNac) in other molecules, the lectin itself does not contain any carbohydrate.  Please see page 231 of the following paper: R. Banerjee, et al., "Crystal structure of peanut lectin, a protein with an unusual quaternary structure".  Proc. Nat. Acad. Sci., USA, 91, 227-231 (1994).

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  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. How does the storage temperature relate to shipping conditions?

    1 answer
    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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