This product has not been tested specifically for use in fungal staining in plant tissue. However, FITC-labeled wheat germ agglutinin or lectin has been cited in the literature for such applications. Please see the link below to review one such publication:
https://iris.unito.it/bitstream/2318/1740844/1/Carotenuto%20et%20al%202.pdf
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偶联物
FITC conjugate
质量水平
表单
lyophilized powder
效能
<50 μg/mL agglutination activity
组成
Protein, ~50% Lowry
标记范围
~2 mol FITC per mol protein
储存温度
2-8°C
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一般描述
凝集素是碳水化合物结合蛋白。它们无处不在，常见于真菌、植物和动物体内。该蛋白质的二级结构富含β-链，并且在表面上具有碳水化合物结合位点。[1]
应用
用于荧光检测含有 β(1→4)-N-乙酰基-D葡糖胺的糖蛋白
生化/生理作用
WGA 不具有血型特异性，但具有与 N-乙酰基-β-D-葡糖胺残基和 N-乙酰基-β-D-葡糖胺低聚物的亲和性。WGA 不含蛋白结合糖。
凝集素在糖缀合物鉴定、成像和靶向过程中发挥着关键作用。它可用于芯片分析，实现有效的糖组图谱分析。在植物和真菌体内，凝集素可防御病原体/寄生物。凝集素在宿主识别和组织粘附中同样发挥主要作用。[1]
包装
基于蛋白含量进行包装。
外形
含有磷酸盐缓冲盐
制备说明
由亲和纯化凝集素制备偶联物。
相关产品
产品编号
说明
价格
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Robert H Oakley et al.
Science signaling, 12(577) (2019-04-18)
Stress is increasingly associated with heart dysfunction and is linked to higher mortality rates in patients with cardiometabolic disease. Glucocorticoids are primary stress hormones that regulate homeostasis through two nuclear receptors, the glucocorticoid receptor (GR) and mineralocorticoid receptor (MR), both
Novel Chemical Suppressors of Long QT Syndrome Identified by an In Vivo Functional Screen
Peal DS, et al.
Circulation (2011)
Rui Guo et al.
Scientific reports, 7(1), 8703-8703 (2017-08-20)
Diabetes is a major risk factor for cardiovascular disease and the lysosomal cysteine protease cathepsin K plays a critical role in cardiac pathophysiology. To expand upon our previous findings, we tested the hypothesis that, knockout of cathepsin K protects against
Yihua Bei et al.
Journal of cellular and molecular medicine, 21(8), 1648-1655 (2017-03-18)
The adult heart retains a limited ability to regenerate in response to injury. Although exercise can reduce cardiac ischaemia/reperfusion (I/R) injury, the relative contribution of cardiac cell proliferation including newly formed cardiomyocytes remains unclear. A 4-week swimming murine model was
Sarah Aileen Lewis et al.
PloS one, 13(8), e0201986-e0201986 (2018-08-14)
Tissues with high-energy demand including the heart are rich in the energy-producing organelles, mitochondria, and sensitive to mitochondrial dysfunction. While alterations in mitochondrial function are increasingly recognized in cardiovascular diseases, the molecular mechanisms through which changes in mitochondria lead to
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Please is this product use in staining Arbuscular mycorrhizal fungi in root, for confocal microscopy examination?
1 answer-
Helpful?
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Does Sigma provide a stain to visualize cell borders for human Cardiomyocytes derived from the GM23338 iPSC cell line?
1 answer-
There is no recommended stain for cells from this type of cell line. However, for visualizing typical cardiomyocyte cell borders, one may consider L4895, as referenced by Vanhoutte D et al, Nat Commun. 2021 Jun 24;12(1):3928. L4895 is Sigma-Aldrich Lectin from Triticum vulgaris (wheat). The article titled "Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy" in Nat Commun. 2021 Jun 24;12(1):3928 may serve as a point of reference.
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