Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
推荐产品
生物来源
Arachis hypogaea (peanut)
质量水平
偶联物
peroxidase conjugate
表单
lyophilized powder
peroxidase activity
≥20 units/mg protein
技术
western blot: suitable
储存温度
−20°C
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应用
通用 Western 印迹法方案：
- 糖蛋白样本量：500 ng
- 凝集素浓度：0.1 μg/mL
- 每个泳道加入500 ng糖蛋白样品
- 运行 4-20％Bis-Tris SDS 页面凝胶
- 将凝胶转移至 PVDF 膜上
- 用 RTA 缓冲液（R0278 Sigma）在室温下封闭膜 1 小时
- 在室温下用 RIPA 缓冲液将 HRP 凝集素以 0.1ug / ml 孵育 2 小时
- 用 25ml RIPA 缓冲液洗涤膜 5×5 分钟
- 使用化学发光底物（CPS1-120）进行检测
生化/生理作用
花生凝集素不会凝集正常的人类红细胞，但会强烈凝集神经氨酸酶处理的红细胞。花生凝集素具有与人血清中的抗 T 抗体相似的有效抗 T 活性。凝集素可用于区分人淋巴细胞亚群。
单位定义
在 20°C、pH 值为 6.0 条件下，1 个单位的邻苯三酚在 20 s 内会形成 1 mg 红桔酚。
外形
含柠檬酸钠
制备说明
采用改进的已发表方法[1]，通过过氧化物酶 (P8375) 制备，有利于低分子量偶联物。通过亲和层析缀合后再纯化。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Matthew R Kudelka et al.
Nature methods, 13(1), 81-86 (2015-12-01)
Protein O-glycosylation has key roles in many biological processes, but the repertoire of O-glycans synthesized by cells is difficult to determine. Here we describe an approach termed Cellular O-Glycome Reporter/Amplification (CORA), a sensitive method used to amplify and profile mucin-type
Timothy J Powell et al.
The Journal of general virology, 100(3), 431-445 (2019-02-05)
A non-replicating form of pseudotyped influenza virus, inactivated by suppression of the haemagglutinin signal sequence (S-FLU), can act as a broadly protective vaccine. S-FLU can infect for a single round only, and induces heterotypic protection predominantly through activation of cross-reactive
Ting-Hsuan Chen et al.
Methods in molecular biology (Clifton, N.J.), 2248, 139-153 (2020-11-14)
Virus-like particle (VLP) technology is an alternative platform for developing vaccines to combat seasonal and pandemic influenza. Influenza VLPs are non-infectious nanoparticles that can elicit effective vaccine immunogenicity in hosts. B-cell-activating factor (BAFF, or BLyS) and a proliferation-inducing ligand (APRIL)
Jessica Tan et al.
Journal of virology, 96(2), e0142121-e0142121 (2021-10-21)
The public health burden caused by influenza virus infections is not adequately addressed with existing vaccines and antivirals. Identifying approaches that interfere with human-to-human transmission of influenza viruses remains a pressing need. The importance of neuraminidase (NA) activity for the
Anna Iervolino et al.
PloS one, 10(3), e0119142-e0119142 (2015-03-24)
Dicer is a crucial enzyme for the maturation of miRNAs. Mutations in the Dicer gene are highly associated with Pleuro Pulmonary Blastoma-Family Dysplasia Syndrome (PPB-FDS, OMIM 601200), recently proposed to be renamed Dicer syndrome. Aside from the pulmonary phenotype (blastoma)
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What is the Department of Transportation shipping information for this product?
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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Is the peanut lectin (Lectin from Arachis hypogaea) a glycoprotein? If so, what is the carbohydrate content?
1 answer-
Peanut lectin is not a glycoprotein. Although peanut lectin binds specifically to a particular carbohydrate (beta-gal(1-->3)galNac) in other molecules, the lectin itself does not contain any carbohydrate. Please see page 231 of the following paper: R. Banerjee, et al., "Crystal structure of peanut lectin, a protein with an unusual quaternary structure". Proc. Nat. Acad. Sci., USA, 91, 227-231 (1994).
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