Hyaluronidase is soluble in water, however most aqueous buffers will be suitable. For testing purposes the bovine enzyme is reconstituted in 20 mM sodium phosphate buffer, with 77 mM sodium chloride, 0.01% BSA, pH 7.0. Solutions are stable up to 1 month refrigerated at 4 deg. C. For long term storage, maintain a concentration of >1 mg/mL, aliquot and freese at - 20 deg. C. Avoid repeated freeze/thaw cycles.
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500 MG
$995.00
1 G
$1,320.00
About This Item
CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54
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单位定义
在 37 ºC、pH 5.35 条件下，一单位每分钟可降解 0.75 μg 多糖透明质酸（通过比浊吸光度测量(λ: 600nm)，与 BSA 复合 45 分钟后测量）。
其他说明
查看更多用于复合糖分析的酶的信息，请访问 www.sigma-aldrich.com/enzymeexplorer
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
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商品
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
实验方案
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
在测定透明质酸酶活性时，使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下，一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。
相关内容
A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.
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