- Plasticity of Human Meniscus Fibrochondrocytes: A Study on Effects of Mitotic Divisions and Oxygen Tension.
Plasticity of Human Meniscus Fibrochondrocytes: A Study on Effects of Mitotic Divisions and Oxygen Tension.
Scientific reports (2017-09-25)
Yan Liang, Enaam Idrees, Stephen H J Andrews, Kirollos Labib, Alexander Szojka, Melanie Kunze, Andrea D Burbank, Aillette Mulet-Sierra, Nadr M Jomha, Adetola B Adesida
PMID28939894
摘要
Meniscus fibrochondrocytes (MFCs) may be the optimal cell source to repair non-healing meniscus injuries using tissue engineering strategies. In this study, we investigated the effects of mitotic divisions and oxygen tension on the plasticity of adult human MFCs. Our assessment techniques included gene expression, biochemical, histological, and immunofluorescence assays. MFCs were expanded in monolayer culture with combined growth factors TGFβ1 and FGF-2 (T1F2) under normoxia (21% O
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