We were trying to use this as a protein standard and found it to be a heterogeneous mess. Not sure what all is in here, but its a lot more than betagalactosidase
G6008
β 半乳糖苷酶 来源于大肠杆菌
β-Galactosidase from Escherichia coli
1 of 1 reviewers received a sample product or took part in a promotion
Grade VI, lyophilized powder, ≥250 units/mg protein
别名:
β-D-半乳糖苷半乳糖水解酶, 乳糖酶
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国内现货，预计发货时间2025年4月07日详情
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1000 UNITS
$147.00
5000 UNITS
$434.00
10000 UNITS
$744.00
About This Item
$147.00
国内现货，预计发货时间2025年4月07日详情
新价格，新优惠！
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一般描述
β-半乳糖苷酶是由四个相同亚基组成的四聚体，每个亚基为135,000 Da。它是一种含巯基的酶，每个亚基含有19个半胱氨酸残基。[1]
生化/生理作用
β-半乳糖苷酶可将乳糖切割成单糖成分（葡萄糖和半乳糖）。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。
β-半乳糖苷酶将乳糖切割成单糖成分，即葡萄糖和半乳糖。它还在反馈环中催化葡萄糖的转糖基化，生成β-半乳糖苷酶的诱导物——异乳糖[4]。
物理属性
四聚体的分子量为465 kDa（每个亚基116.3 kDa）
单位定义
在 37 °C，pH 7.3 条件下，一个单位每分钟可将 1.0μmole 邻硝基苯基β-D-半乳糖苷水解为邻硝基苯酚和 D-半乳糖。
外形
部分纯化；含有Tris缓冲盐、氯化镁、DL-二硫苏糖醇和 2-巯基乙醇
底物
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
David P Klebl et al.
Frontiers in molecular biosciences, 9, 945772-945772 (2022-08-23)
Advances in single particle cryo-EM data collection and processing have seen a significant rise in its use. However, the influences of the environment generated through grid preparation, by for example interactions of proteins with the air-water interface are poorly understood
K Kato et al.
Journal of immunology (Baltimore, Md. : 1950), 116(6), 1554-1560 (1976-06-01)
1. A method for the conjugation of the Fab' fragment of rabbit IgG with beta-D-galactosidase from Escherichia coli is described. The method consists of two main steps: treatment of the Fab' fragments containing sulfhydryl groups with excess N,N'-o-phenylenedimaleimide, to introduce
PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.
G R CRAVEN et al.
The Journal of biological chemistry, 240, 2468-2477 (1965-06-01)
D C Young et al.
Analytical biochemistry, 215(1), 24-30 (1993-11-15)
Bacterial beta-galactosidase is one of several reporter enzymes used in studying the transcriptional activity of eukaryotic promoters. Although it is one of the easiest and least expensive enzymes to assay, its use has been limited because of its low sensitivity
Orit Redy-Keisar et al.
Nature protocols, 9(1), 27-36 (2013-12-07)
This protocol describes the synthesis of modular turn-ON QCy7-based probes for the detection of biologically relevant analytes, such as hydrogen peroxide, ubiquitous sulfhydryl and β-galactosidase. The probes presented herein are prepared through a simple procedure that involves the preliminary alkylation
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