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59767

Sigma-Aldrich

氯酚红-β-D-吡喃半乳糖苷

β-galactosidase, ≥90% (HPLC), powder or crystals

别名:

CPRG, 氯酚红-β-D-半乳糖苷

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25 MG
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$230.00

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25 MG
$74.80
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About This Item

经验公式（希尔记法）:
C25H22Cl2O10S
CAS号:
99792-79-7
分子量:
585.41
MDL编号:
MFCD05863726
UNSPSC代码:
12352204
PubChem化学物质编号:
57651696
NACRES:
NA.83

$74.80

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产品名称

氯酚红-β-D-吡喃半乳糖苷, ≥90% (HPLC)

方案

≥90% (HPLC)

表单

powder or crystals

溶解性

water: 20 mg/mL, clear, orange to very deep red

储存温度

−20°C

SMILES字符串

OC[C@H]1O[C@@H](Oc2ccc(cc2Cl)C(=C3/C=CC(=O)C(Cl)=C3)\c4ccccc4S(O)(=O)=O)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C25H22Cl2O10S/c26-15-9-12(5-7-17(15)29)21(14-3-1-2-4-20(14)38(33,34)35)13-6-8-18(16(27)10-13)36-25-24(32)23(31)22(30)19(11-28)37-25/h1-10,19,22-25,28,30-32H,11H2,(H,33,34,35)/b21-12-/t19-,22+,23+,24-,25-/m1/s1

InChI key

YOUWVNCQJOMMEU-AETRGLENSA-N

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一般描述

氯酚红-β-D-吡喃半乳糖苷(CPRG)是一种长波长黄色[1]染料，常用于比色检测。[2]它可作为 β-半乳糖苷酶 (β-gal) 的底物，半乳糖苷酶可水解CPRG 形成氯酚红（紫色）。该产品通过测量其在 570 nm 处的吸光度进行定量检测。[1]CPRG 用于鉴定粪便大肠菌群。它的灵敏度比邻-硝基苯基-β-半乳糖苷（ONPG）等显色剂高10倍左右。CPRG还具有很高的快速转化率。[2]

应用

氯酚红-β-D-吡喃半乳糖苷已被用作 β-半乳糖苷(β-ga）的底物：
  • 用于靶RNA检测的基于纸的物细胞蛋白质表达体系[1]
  • 采用lacZ输出基因进行多个临床相关突变的比色检测[3]
  • 用于细菌定量的比色测定[4]

其他说明

Measures the expression levels of β-galactosidase in transfected cells. The lacZ gene is a popular reporter gene in transfection reactions because its product, β-galactosidase, is a very stable enzyme.citationcitation
测量转染细胞中 β-半乳糖苷酶的表达水平。lacZ 基因是一种在转染反应中常用的报告基因，因为它的产物 β-半乳糖苷酶是一种非常稳定的酶。[5][6]

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCL5523
BCCH9407
BCCF5075
BCCB4668
BCBZ7020

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J H Felgner et al.
The Journal of biological chemistry, 269(4), 2550-2561 (1994-01-28)
The application of cationic liposome reagents has advanced DNA and mRNA transfection research in vitro, and data are accumulating which show their utility for in vivo gene transfer. However, chemical structure-activity data leading to a better mechanistic understanding of their
Fan Hong et al.
Cell, 180(5), 1018-1032 (2020-02-29)
The ability to identify single-nucleotide mutations is critical for probing cell biology and for precise detection of disease. However, the small differences in hybridization energy provided by single-base changes makes identification of these mutations challenging in living cells and complex
Monpichar Srisa-Art et al.
Analytical chemistry, 90(1), 1035-1043 (2017-12-07)
Salmonella causes over a million foodborne illnesses per year in the United States resulting in more hospitalizations and deaths than any other foodborne bacterial pathogen. To help prevent outbreaks, a rapid, portable, sensitive, and reliable method for onsite detection of
Generation of antigen-specific, lacZ-inducible T-cell hybrids.
S Malarkannan et al.
Methods in molecular biology (Clifton, N.J.), 156, 265-272 (2000-11-09)
C Vega et al.
Parasitology research, 95(4), 296-298 (2005-02-01)
We have developed a new pharmacological screening assay for epimastigotes of Trypanosoma cruzi (clone CL-B5) that express the Escherichia coli LacZ gene. The assay is based on determining the activity of the cytoplasmic beta-galactosidase released into the culture on membrane
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