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Merck

G5160

Sigma-Aldrich

β 半乳糖苷酶 来源于米曲霉

≥8.0 units/mg solid

别名:

β-D-半乳糖苷半乳糖水解酶, 乳糖酶 来源于米曲霉

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

solid

比活性

≥8.0 units/mg solid

包含

dextrin as extender

儲存溫度

−20°C

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一般說明

ββ-半乳糖苷酶属于糖苷水解酶clan A(GH-A)家族的一员,广泛存在于包括原核生物和真核生物的微生物中。

應用

β-半乳糖苷酶(来源于米曲霉Aspergillus oryzae)已用于:
  • 体外核磁共振(NMR)
  • 作为β-半乳糖苷酶分析标准品,测定细胞相关的β-半乳糖苷酶浓度
  • 修饰突变滑液细胞(slime cell)表面的糖残基以研究粘附性
  • 乳糖酶促水解实验
  • 反相(RP)吸附实验
  • 乳清乳糖水解

生化/生理作用

ββ-半乳糖苷酶作用于二糖、糖缀合物和多糖末端的β-D-半乳糖基。它可用于介导转糖基反应,比如低聚半乳糖(GOS)合成。ββ-半乳糖苷酶可用于糖生物学和生物技术应用。
β-半乳糖苷酶切割乳糖成单糖成分,葡萄糖和半乳糖。它还能催化葡萄糖转糖基化形成异乳糖,在反馈回路中作为β-半乳糖苷酶的诱导剂。

單位定義

一单位每分钟水解1.0 μmole乳糖,pH4.5,30℃。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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