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應用
β-半乳糖苷酶用于咪唑并吡咯烷糖的酶促合成。
生化/生理作用
β-半乳糖苷酶可将乳糖切割成单糖成分(葡萄糖和半乳糖)。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。
物理性質
四聚体的分子量为465 kDa(每个亚基116.3 kDa)
單位定義
在 37°C、pH 7.3 条件下,1 个单元每分钟水解 1.0 μmol 邻硝基苯基 β-D-半乳糖苷,生成邻硝基苯酚和 D-半乳糖。
外觀
混悬于 50% 甘油、5 mM Tris 缓冲盐、5 mM 氯化镁、0.5 mM DTT、0.5 mM 巯基乙醇中,pH 7.4。
其他說明
如果该酶被冷冻,可能发生酶活性丧失。这种酶在 50% 甘油溶液中提供,如果在-20°C 下储存,不会冷冻。不建议将此酶制剂保存在超冷冰箱中 (-60 至-100° C)。
基底
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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