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F6057

Sigma-Aldrich

Fluoroshield™ 封固剂（含DAPI）

liquid

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20 ML
$183.40

$183.40

目录价$262.00节省 30%

预计发货时间2025年2月03日详情

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20 ML
$183.40

About This Item

分類程式碼代碼:
12171500
NACRES:
NA.47

$183.40

目录价$262.00节省 30%

预计发货时间2025年2月03日详情

获取大包装报价

产品名称

Fluoroshield™ 封固剂（含DAPI）, histology mounting medium

品質等級

200

形狀

liquid

成份

Sodium Azide, ≤1% (also contains other ingredients)
Tris-HCl

技術

immunofluorescence: suitable

pH值範圍

7.9-8.3

溶解度

water: soluble

螢光

λex 360 nm; λem 460 nm

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

2-8°C

相关类别

一般說明

含DAPI的Fluoroshield是一种含有4′,6-二氨基-2-苯基吲哚(DAPI)的水固载介质，也是一种核反染剂，可用作蓝色荧光DNA探针。它是一种抗褪色剂，用于保存组织和细胞涂片的荧光，通过防止快速光漂白和最小化眨眼介导的现象。(1)用于原位杂交技术或其他需要DNA染色荧光的方法。DAPI的激发波长为360nm，发射波长为460nm，产生蓝色荧光。RNA也可以采用DAPI染色。

應用

Fluoroshield™封固剂（含DAPI）可用于 ：
  • 用作免疫细胞化学中的复染剂，可标记神经祖细胞的细胞核[1]
  • 用作免疫荧光研究中心脏组织部分的组织学封固剂[2]
  • 用作细胞核检测中免疫组化染色心脏部分的封固溶液[3]

法律資訊

FluoroShield is a trademark of ImmunoBioScience Corp.

相關產品

产品编号
说明
价格

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000411200
0000411200
0000405985
0000403233
0000391225

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Extracellular matrix-derived extracellular vesicles promote cardiomyocyte growth and electrical activity in engineered cardiac atria
An M, et al.
Biomaterials, 146(1), 49-59 (2017)
Edgar T Hoorntje et al.
PloS one, 13(8), e0203078-e0203078 (2018-08-31)
Likely pathogenic/pathogenic variants in genes encoding desmosomal proteins play an important role in the pathophysiology of arrhythmogenic right ventricular cardiomyopathy (ARVC). However, for a substantial proportion of ARVC patients, the genetic substrate remains unknown. We hypothesized that plectin, a cytolinker
miR-210 suppresses BNIP3 to protect against the apoptosis of neural progenitor cells
Wang F, et al.
Stem Cell Research, 11(1), 657-667 (2013)
S Hamada et al.
Histochemistry, 79(2), 219-226 (1983-01-01)
DNA-DAPI complexes emit strong bluish white fluorescence when excited by ultraviolet light so that even very small amounts of DNA such as those in mitochondria, chloroplasts, and virus particles can be visualized. Moreover, the staining procedure with DAPI is very
Maria L Spletter et al.
eLife, 7 (2018-05-31)
Muscles organise pseudo-crystalline arrays of actin, myosin and titin filaments to build force-producing sarcomeres. To study sarcomerogenesis, we have generated a transcriptomics resource of developing Drosophila flight muscles and identified 40 distinct expression profile clusters. Strikingly, most sarcomeric components group
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Questions

1–10 of 15 Questions  

  1. How is Fluoroshield (F6057) used with DAPI?

    1 answer

    1. Fluoroshield with DAPI is an aqueous mounting medium designed to preserve the fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of various fluorescent dyes. Additionally, it retains fluorescence during prolonged storage at 4°C in the dark. Importantly, the medium does not contain phenylenediamine, which can destroy immunofluorescence of certain dyes. The mounting medium is fortified with DAPI, a counter-stain for DNA, intended for use in situ hybridization techniques or other methods requiring fluorescence of DNA staining. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI. The intended use of this product includes immunofluorescence and confocal microscopy.

      Reagent:
      Fluoroshield with DAPI is a ready-to-use mounting medium.

      Storage:
      It should be stored at 2-8°C, protected from light, and should not be frozen.

      Procedure:
      - Bring the vial to room temperature.
      - Rinse the slide to be mounted with distilled or deionized water, remove excess water by touching the edges of the slide on a paper towel, and place the slides on a flat surface away from light.
      - Apply 3-4 drops of mounting medium directly on top of the specimen and spread it out evenly, ensuring the tissue is not touched. Excess medium can be removed by touching the edges of the slide on a paper towel.
      - Let it stand at room temperature for about 5 minutes.
      - Apply a cover slip, carefully avoiding air bubbles. If a coverslip is not used, air bubbles will appear in a few days.
      - For long-term storage, it is recommended to store the slide in the dark at 2-8°C.

      Removal of Coverslip:
      The coverslip can be removed before sealing the edges. Soak the slide in warm (37°C) water for a few minutes, carefully and slowly moving.

      Helpful?

  2. What could be the reason for the occurrence of a white film after curing, particularly when the coverslips are slightly wet with PBS?

    1 answer

    1. The following recommendations are provided:

      •If there is tissue attached to glass slides, it is recommended to use a Kimwipe to carefully dry the slide around the tissue section.
      •If cells are on a coverslip, removing excess water from the coverslip by placing it on its end and then using a paper towel to wick away excess moisture is suggested.
      •The possibility that as the slides dry, salts from the PBS are evaporating and leaving a white residue or film is considered.

      Helpful?

  3. What are the procedures for solubilizing and using Fluoroshield with DAPI (F6057)?

    1 answer

    1. Fluoroshield with DAPI is a solution that is ready to use and does not require solubilization. It is an aqueous mounting medium designed to preserve the fluorescence of tissue and cell smears. The mounting medium is fortified with DAPI, a counter-stain for DNA, and is intended for use in situ hybridization techniques, immunofluorescence and confocal microscopy.

      Helpful?

  4. What are the potential implications of storing a bottle of F6057-20 at -20°C for 2 days by mistake?

    1 answer

    1. It is suggested to allow the bottle to thaw. Once thawed, the bottle should be inverted several times to thoroughly mix its contents. If the solution looks normal and does not contain any precipitate or particulate matter, it is expected that the mounting media is suitable for use.

      Helpful?

  5. Is sealing a 96-well plate necessary in the final step of ICC with Fluoroshield™ with DAPI, as done with coverslips using clear nail polish?

    1 answer

    1. Fluoroshield with DAPI is designed for use with glass slides or coverslips intended for analysis using a fluorescent microscope. It is not commonly used for coverslipping 96 well plates. There are no known individual, small, round coverslips for each of the 96 wells, nor are there large clear glass plates to fit over the top of a 96 well plate. If such glass plates are available, it is not known that such a product exists.

      Helpful?

  6. what is the refractive index？

    1 answer

    1. The refractive index is not determined internally for this product. However, the supplier reports that it is 1.364 ± 0.002 of the product in solution.

      Helpful?

  7. Is Fluoroshield with DAPI (F6047) compatible for high-maginification microscopy or is it recommended to use hard-setting medium?

    1 answer

    1. This product has been used for cell imaging. Please see the following protocols and review the pee-reviewed articles:

      https://www.sigmaaldrich.com/search/f6057?focus=papers&page=1&perpage=30&sort=relevance&term=f6057&type=citation_search

      https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/cell-counting-and-health-analysis/lentiviral-live-cell-autophagy-lc3

      https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/imaging-analysis-and-live-cell-imaging/lentiviral-live-cell-cytoskeleton

      https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/3d-cell-culture/3d-organoid-culture

      Helpful?

  8. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  10. How do I use Fluoroshield (Product Nos. F6182, F5932, F6057)?

    1 answer

    1. 1. Bring the vial to room temperature. 2. Rinse slide to be mounted with DISTILLED OR DEIONIZED WATER, then touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface away from light. 3. Turn the vial upside down and remove any air bubbles. 4. Apply 3-4 drops directly of mounting medium on top of the specimen and spread out evenly by tilting the slide back and forth or spread evenly with a 0.2 ml plastic pipette tip, making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide on paper towel. 5. Let stand at room temperature for about 5 minutes. 6. Apply cover slip, carefully avoiding air bubbles. 7. The specimen is ready for visualization under microscope. 8. One can seal the edges of cover slip with nail polish or any organic medium. If coverslip is not used air bubbles will appear in few days. 9. For long term storage it is recommended that the slide be stored in the dark at 2-8°C.

      Helpful?

1–10 of 15 Questions  

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