One bottle of the product makes 1 liter of working solution. The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 Liter with 800 mL water.
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一般說明
重構
用 800 mL 水稀释至 1 L 后，混悬液将含有 0.1% (w/v) 亮蓝 G、0.29 M 磷酸和 16% 饱和硫酸铵。
分析報告
已在 SDS-PAGE 上检测了本品的适用性。
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说明
价格
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
儲存類別代碼
8B - Non-combustible corrosive hazardous materials
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Roy Auty et al.
The Journal of biological chemistry, 279(48), 49973-49981 (2004-09-28)
The basal transcription factor TFIID is composed of the TATA-binding protein (TBP) and 14 TBP-associated factors (TAFs). Although TBP alone binds to the TATA box of DNA and supports basal transcription, the TAFs have essential functions that remain poorly defined.
Julie Ducreux et al.
Bioconjugate chemistry, 19(10), 2088-2094 (2008-09-24)
PEGylation of antibodies is known to increase their half-life in systemic circulation, but nothing is known regarding whether PEGylation can improve the inhibitory potency of antibodies against target receptors. In this paper, we have examined this question using antibodies directed
Purification of Active TFIID from Saccharomyces cerevisiae EXTENSIVE PROMOTER CONTACTS AND CO-ACTIVATOR FUNCTION
Auty R, et al.
The Journal of biological chemistry, 279(48), 49973-49981 (2004)
A multi-laboratory evaluation of a common in vitro pepsin digestion assay protocol used in assessing the safety of novel proteins
Thomas K, et al.
Regulatory Toxicology and Pharmacology, 39(2), 87-98 (2004)
Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels
Dyballa N and Metzger S
Journal of Visualized Experiments (2009)
实验方案
Brilliant Blue G-Colloidal Concentrate stains proteins in IEF, PAGE, and SDS-PAGE gels after electrophoresis, enhancing sensitivity with protein fixation.
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How would you recommend that I reconstitute Product B2025, Brilliant Blue G?
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What is the detection limit of Product B2025, Brilliant Blue G?
1 answer-
The detection limit for proteins is about 50 ng of protein. However, proteins pick up stains differently so this is a general description and may not apply to all proteins
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How is Product B2025, Brilliant Blue G, better than other Coomassie Blue dyes?
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The major advantage of the colloidal form is that it gives less background staining that the "regular" non-colloidal Coomassie Brilliant Blue G and Brilliant Blue R stains. Sensitivity can be markedly increased by double staining, first with Coomassie Brilliant Blue G Colloidal, then Rapid Silver Stain.
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What is the solution stability of Product B2025, Brilliant Blue G?
1 answer-
Once diluted the solution should be stable indefinitely. It should be stored in the refrigerator and shaken well before each use. The solution tends to precipitate on standing and heat accelerates precipitation. Therefore, do not leave the bottle at room temperature overnight.
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Can you use Product B2025, Brilliant Blue G, with nitrocellulose?
1 answer-
Use with nitrocellulose is not recommended, due to the methanol.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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