This product is provided as a 5000X concentration in DMSO. The specific concentration of this dye is considered proprietary.
$122.00
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儲存期限
≥6 mo. (when stored desiccated and protected from light at room temperature, 4 °C or 20 °C.)
品質等級
技術
protein staining: suitable
螢光
λex 300,470 nm; λem 570 nm
相关类别
一般說明
SYPRO Orange与SYPRO Red相似，但它颜色更亮一些，背景荧光略高。 对于使用激光激发凝胶扫描仪的用户，推荐使用一种基于氩离子激光的SYPRO Orange仪器。 该染料可由紫外线或宽带照明光有效激发。它不适用于印迹或IEF凝胶中蛋白的染色，并在2维凝胶染色中显示出较低的灵敏度。
SYPRO Orange为：
SYPRO Orange为：
- 高灵敏度。 该染色剂可检测每minigel条带中1-2ng的蛋白质，比考马斯亮蓝或银染色更灵敏。
- 快速。 在不到1小时内完成染色。
- 简单。 电泳后，对凝胶进行染色、冲洗并拍照；不需要固定或脱色步骤
- 与标准实验室设备兼容。 染色蛋白可以用标准的300纳米紫外透照仪或激光扫描仪来观察。
- 物美价廉。 SYPRO Orange染色法较银染法便宜，并且所需时间短。
- 不同蛋白间变化小。 染料与蛋白质周围的SDS包被层相互作用，使不同类型的蛋白质之间的染色比考马斯或银染色更一致。
- 对蛋白的选择性。 SYPRO Orange检测的蛋白质小至6.5 kDa，不染色核酸或脂多糖。 它染色糖基化蛋白。
- 较宽的检测线性范围。 染色条带的荧光强度与超过3个数量级的蛋白量呈线性相关(比考马斯染色或银染色范围更广)。
注意
SYPRO储液应在室温、4°C或20°C下干燥保存并避光。如果储存得当，这些储液可以稳定使用6个月到1年。使用缓冲液或醋酸溶液稀释后的染色试剂可以储存在非常干净、无去污剂的玻璃瓶或塑料瓶中，在4℃下避光保存至少3个月。
法律資訊
SYPRO is a registered trademark of Life Technologies
相關產品
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
188.6 °F - closed cup
閃點（°C）
87 °C - closed cup
Influence of GTP on system specific chaperone - Twin arginine signal peptide interaction.
Cherak SJ and Turner RJ
Biochemical and Biophysical Research Communications, 465, 753-757 (2015)
Ruth Cohen-Khait et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(52), 14982-14987 (2016-12-14)
Protein-protein interactions occur via well-defined interfaces on the protein surface. Whereas the location of homologous interfaces is conserved, their composition varies, suggesting that multiple solutions may support high-affinity binding. In this study, we examined the plasticity of the interface of
Algal lectin binding to core (a1-6) fucosylated N-glycans: structural basis for specificity and production of recombinant protein.
do Nascimento AS, et al.
Glycobiology, 25, 607-616 (2015)
Glu121-Lys319 salt bridge between catalytic and N-terminal domains is pivotal for the activity and stability of Escherichia coli aminopeptidase N.
Gumpena R, et al.
Protein Science, 21, 727-736 (2012)
M Murshida Mahbub et al.
Mobile DNA, 8, 16-16 (2017-11-21)
R2 elements are a clade of early branching Long Interspersed Elements (LINEs). LINEs are retrotransposable elements whose replication can have profound effects on the genomes in which they reside. No crystal or EM structures exist for the reverse transcriptase (RT)
商品
为满足蛋白分析的多样化需求，Sigma提供多种蛋白质可视化（染色）试剂。EZBlue™和ProteoSilver™专为蛋白质组学而设计，即使在传统的PAGE形式中依然表现出色。
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
实验方案
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。
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サイプロオレンジ s5692 50ul の濃度は5mMですか？
1 answer-
Helpful?
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It's used for Protein quantification assay?
1 answer-
This reagent is suitable for the detection of proteins. However, protein quantitation typically utilizes other methods, as described in the graphic below. Please see the following link to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/161/599/s5692dat.pdfHelpful?
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1. Is the SYPRO Orange Protein Gel Stain a 5,000x concentrate?
2. Is there a protocol available for using the SYPRO Orange protein stain for a thermal denaturation assay?1 answer-
The SYPRO Orange Protein Gel Stain is indeed provided as a 5000X concentrated solution in DMSO.
Regarding a protocol for using the SYPRO Orange protein stain for a thermal denaturation assay, the product information is focused on gels. While the product is not specifically tested for this application,Helpful?
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