The source information will be listed on the lot specific Certificate of Origin. Please navigate to the DOCUMENTATION section of the Product Detail Page to access a certificate under the ‘Certificate of Origin’ tab: https://www.sigmaaldrich.com/product/sigma/a7888#product-documentation
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生物源
bovine
品質等級
等級
RIA grade
化驗
≥96%
形狀
lyophilized powder
品質
suitable for RIA
分子量
~66 kDa
純化經由
heat shock fractionation
包裝
poly bottle of
來源
USA origin
技術
blood typing: suitable
損耗
≤5%
pH值
5.2
溶解度
water: soluble (40 mg/ml)
適合性
suitable for insulin RIA procedures
UniProt登錄號
異物活動
BT virus, none detected
VSV virus, none detected
儲存溫度
2-8°C
基因資訊
bovine ... ALB(280717)
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一般說明
牛血清白蛋白（BSA）是一种由每个均带有两个亚结构域的三个结构域所组成的66 kDa蛋白。它是一种α-螺旋、球状且非糖基化的蛋白。[1]
應用
牛血清白蛋白已用于：
- 酶联免疫吸附试验（ELISA）的封闭剂（154）
- 作为窦前卵泡培养的Dulbecco PBS培养基的组分（155）
- 作为卵母细胞授精的改良Tris缓冲培养基（mTBM）的组分（156）
生化／生理作用
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
特點和優勢
- 热休克分级
- 适用于放射免疫分析（RIA）
注意
在某些程序中，可能会产生高达0.1 μ单位/毫克的胰岛素空白。
準備報告
使用热休克分级制备
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
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Low blood phosphate (Pi) reduces muscle function in hypophosphatemic disorders. Which Pi transporters are required and whether hormonal changes due to hypophosphatemia contribute to muscle function is unknown. To address these questions we generated a series of conditional knockout mice
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Bacterial proteins exported to the cell surface play key cellular functions. However, despite the interest to study the localisation of surface proteins such as adhesins, transporters or hydrolases, monitoring their dynamics in live imaging remains challenging, due to the limited
K B Cullberg et al.
Nutrition & diabetes, 4, e113-e113 (2014-03-26)
High levels of free fatty acids (FFA) have been suggested to be one of the underlying mechanisms for adipose tissue (AT) inflammation and dysfunction in obesity. Human AT produces several adipokines including monocyte chemoattractant protein-1 (MCP-1), which are involved in
Enzyme-linked immunosorbent assay (ELISA) and blocking with bovine serum albumin (BSA)?not all BSAs are alike
Xiao Y and Isaacs SN
Journal of Immunological Methods, 384(1-2), 148-151 (2012)
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Is this from bred animal?
1 answer-
Helpful?
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At what concentration is Product A7888, Albumin from bovine serum (BSA), soluble in water?
1 answer-
We have not established an upper limit of solubility for this product in water. At 40 mg/mL, an aqueous solution of this product may light yellow to green and slightly hazy.
Helpful?
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What is the molecular weight of Product A7888, Albumin from bovine serum?
1 answer-
BSA consists of 583 amino acid residues. The molecular weight calculated by NIST is 66429.03. It has also been calculated by GPMAW (General Protein Mass Analysis for Windows) as 66429.94.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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What does RIA grade mean in the description of Product A7888, Albumin from bovine serum?
1 answer-
RIA grade means that this product has been shown suitable for radioimmunoassays.
Helpful?
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