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生物来源
mouse
重组
expressed in mouse cell line
偶联物
alkaline phosphatase conjugate
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
DI-22, monoclonal
表单
buffered aqueous solution
技术
direct ELISA: 1:10,000 using using 20 μg/ml of purified digoxin-BSA for coating and pNPP substrate
direct ELISA: 1:6,000 using using 10 μg/ml of digoxigenin-transferrin for coating and pNPP substrate
dot blot: 1:20,000 using digoxigenin-labeled biomolecules (chemiluminescent)
同位素/亚型
IgG1
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
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一般描述
地高辛是一种强心苷，可用于治疗慢性心力衰竭。抗地高辛碱性磷酸酶单克隆抗体可用于检测地高辛标记的化合物，例如寡核苷酸、抗体和肽。该抗体对地高辛和地高辛标记的化合物具有特异性。该产品与地高辛具有很强的交叉反应性。
特异性
抗体对地高辛具有特异性，且对地高辛具有高亲和力。
免疫原
地高辛-KLH。
应用
巢式PCR产物可与5′端地高辛标记的寡核苷酸探针杂交。 向1 M磷酸盐缓冲液、1％牛血清白蛋白和0.1％吐温20（pH 7.4）中添加1:1000稀释的抗地高辛单克隆抗体碱性磷酸酶（DI-22）进行ELISA分析，以检测探针。使用Sigma Fass-Pnpp底物片剂进行检测。
抗地高辛碱性磷酸酶单克隆抗体适用于直接ELISA（1:10000-1:6000）和斑点杂交（以1:20,000稀释，使用地高辛标记的生物分子）。它还用于酶免疫分析（EIA）。
外形
0.05 M Tris缓冲液，pH 8.0，含1% BSA、50%甘油、1 mM MgCl2和15 mM叠氮化钠
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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