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用途
sufficient for 25 labeling reactions
sufficient for 50 blots
品質等級
製造商／商標名
Roche
環保替代產品特色
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
環保替代類別
, Aligned
儲存溫度
−20°C
一般說明
DIG DNA标记和检测试剂盒是使用便捷的试剂盒，用地高辛-脱氧尿苷三磷酸（dUTP）标记DNA随机引物，通过酶免疫法对杂交产物进行碱解，测定颜色。在此方法中，使用随机寡核苷酸3′-OH末端用作引物，在Klenow聚合酶的作用下合成变性 DNA 的互补 DNA 链。
内容物：
内容物：
- 未标记的对照DNA 1, 100 μg/ml
- 未标记的对照DNA 2, 200 μg/ml
- DNA稀释缓冲液
- DIG标记的对照DNA, 5.2 μg/ml
- 10X 六核苷酸混合物
- 10x dNTP标记混合物
- Klenow 酶，标记等级，2 U/μl
- 抗地高辛-AP结合物，750 U / ml
- NBT/BCIP 浓缩储存液
- 封闭剂
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。本产品为一种安全的化学品。DIG系统是灵敏且具有成本效益的方案，可替代核酸的放射性标记和检测方法。有许多已发表论文证明了DIG系统的通用性，因此，使用放射性标记不再是标记用于杂交的DNA的唯一选择。
特異性
灵敏度和特异性：1 μg模板37℃，1小时培育，标准标记反应会产生260ngDIG-标记DNA，20小时培育会生成780ng。DIG检测灵敏度取决于杂交反应中DIG-标记探针浓度和颜色反应持续时间。
應用
包裝
1个试剂盒包含10种组分。
準備報告
工作浓度：将抗体稀释到1：5000
其他說明
仅用于生命科学研究。不可用于诊断。
仅试剂盒组分
产品编号
说明
- Unlabeled Control DNA 1 100 µg/ml
- Unlabeled Control DNA 2 200 µg/ml
- DNA Dilution Buffer
- DIG-labeled Control DNA 5.2 µg/ml
- Hexanucleotide Mix 10x concentrated
- dNTP Labeling Mixture 10x concentrated
- Klenow Enzyme, Labeling grade 2 U/µl
- Anti-digoxigenin-AP-conjugate antibody 750 U/ml
- NBT/BCIP Concentrated Stock Solution
- Blocking Reagent
查看所有结果 (10)
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
does not flashNot applicable
閃點（°C）
does not flashNot applicable
其他客户在看
Shun Sawatsubashi et al.
Scientific reports, 8(1), 593-593 (2018-01-14)
CRISPR/Cas9-based genome editing has dramatically accelerated genome engineering. An important aspect of genome engineering is efficient knock-in technology. For improved knock-in efficiency, the non-homologous end joining (NHEJ) repair pathway has been used over the homology-dependent repair pathway, but there remains
Jiaqiang Wang et al.
Cell, 175(7), 1887-1901 (2018-12-15)
In early mammalian embryos, it remains unclear how the first cell fate bias is initially triggered and amplified toward cell fate segregation. Here, we report that a long noncoding RNA, LincGET, is transiently and asymmetrically expressed in the nucleus of
Diana C Garcia-Ramon et al.
Microbial biotechnology, 11(2), 302-316 (2017-10-14)
Bacillus pumilus strain 15.1 was previously found to cause larval mortality in the Med-fly Ceratitis capitata and was shown to produce crystals in association with the spore. As parasporal crystals are well-known as invertebrate-active toxins in entomopathogenic bacteria such as
Nasser Shakhssalim et al.
Cancer cell international, 13(1), 120-120 (2013-12-07)
Bladder cancer is a relatively common and potentially life-threatening neoplasm that ranks ninth in terms of worldwide cancer incidence. The aim of this study was to determine deletions and sequence variations in the mitochondrial displacement loop (D-loop) region from the
Hongshun Li et al.
International journal of molecular sciences, 22(11) (2021-06-03)
The vegetative phase transition is a prerequisite for flowering in angiosperm plants. Mulberry miR156 has been confirmed to be a crucial factor in the vegetative phase transition in Arabidopsis thaliana. The over-expression of miR156 in transgenic Populus × canadensis dramatically
商品
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
地高辛（DIG）标记方法和试剂盒适用于DNA和RNA DIG探针、随机引物DNA标记、切口平移标记、5'和3'寡核苷酸末端标记。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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