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Merck

A4018

Sigma-Aldrich

2-羟乙基琼脂糖

别名:

低凝胶温度的琼脂糖, 2-羟乙基琼脂糖

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1 G
$57.60
5 G
$125.00
10 G
$232.00
25 G
$402.00
50 G
$690.00
100 G
$1,240.00

$57.60


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变更视图
1 G
$57.60
5 G
$125.00
10 G
$232.00
25 G
$402.00
50 G
$690.00
100 G
$1,240.00

About This Item

CAS号:
MDL编号:
UNSPSC代码:
41105317
NACRES:
NA.21

$57.60


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生物来源

algae (marine)

质量水平

表单

powder

技术

cell culture | mammalian: suitable

EEO

≤0.10

转变温度

congealing temperature 26-30 °C

凝胶强度

≥200 g/cm2 (1% gel)

SMILES字符串

O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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一般描述

琼脂糖是一种从琼脂或含琼脂的海藻中提取的聚合物。这种纯化线性半乳糖水胶体包括由 α-(1→3) 和β-(1→4)糖苷键连接的交替共聚物D-半乳糖和3,6-内醚-L-半乳糖单元。[1]琼脂糖具有高度的生物相容性,并具有不同的力学和扩散特性。[2]琼脂糖可用作凝胶剂,用于电泳分离核酸。低熔点或低冻胶化温度的琼脂糖是由琼脂糖羟乙基化制备而成。它通常用于分离已分离的DNA片段。与标准琼脂糖凝胶相比,相同大小的DNA片段在低熔点琼脂凝胶中移动需要更长的时间。[3]

应用

凝胶表现出优异的透明度,特别适用于制备含有热不稳定材料的培养基。推荐用于琼脂糖微球的制备。
使用低凝胶温度琼脂糖:
  • 在活体成像实验期间固定斑马鱼胚胎[4]
  • 用作培养牛髓核(NP)细胞的确定成分培养基的组成部分[5]
  • 作为水凝胶,应用于牙髓再生的支架材料中[6]
  • 以颗粒的形式测定丙烯酸乳液膜的表面pH值和电导率[7]

质量

低凝胶温度衍生物,具有独特的凝胶特性。

分析说明

以下是与我们的琼脂糖相关的属性列表:
含硫量 -用作纯度指标,因为硫酸盐是主要的离子基团。
凝胶强度 -必须施加于凝胶上使其断裂的力。
凝胶点 -液体琼脂糖溶液冷却时形成凝胶的温度。 琼脂糖溶液在液体至凝胶转变过程中表现出迟滞现象,即凝胶点与熔融温度不同。
电内渗(EEO) -液体通过凝胶的运动。琼脂糖凝胶中的阴离子基团附着在基体上不能移动,但游离的反阳离子可以向基体的阴极迁移,从而产生EEO。 由于生物聚合物的电泳运动通常是向阳极方向的,由于EEO的内部对流,EEO可以干扰这些分离。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Questions

1–3 of 3 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. In order to have sterile Low Melting Agarose, once the package has already been opened outside of sterile conditionds, should I filter it and then lyophilize or should I use an autoclave?

    1 answer
    1. Agarose solutions can be autoclaved.

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