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Merck

10901393001

Roche

糖原

from mussels

别名:

糖原

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1 ML
$293.00

$293.00


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1 ML
$293.00

About This Item

UNSPSC代码:
41116100

$293.00


请联系客服了解存货情况

质量水平

表单

solution

包装

pkg of 1 mL (20 mg)

制造商/商品名称

Roche

储存温度

−20°C

应用

该制剂可用作核酸(DNA或RNA)沉淀的载体。[1][2]作为惰性材料,它可以代替tRNA或超声处理的DNA。
20 μg糖原(1 μl溶液)可以从1 ml的体积中沉淀出pg量的DNA或RNA。
在一个典型实验中,将5 pg [3H]标记的小牛胸腺DNA溶解于含1 mM EDTA、0.4 M LiCl的500 μl 10 mM Tris-HCl(pH 8.0)中。加入1 μl糖原溶液(20 μg糖原)作为载体,然后在-15至-25 °C下用1.2 ml乙醇沉淀,并在-15至-25 °C下保存3小时。 离心(12 000×g 10分钟)后,总放射性存在于沉淀物中。不添加糖原,DNA不会沉淀。

特点和优势

糖原具有特殊的分子生物学性质,是核酸制剂中的惰性载体。

内容
水溶液,20 mg/ml

分析说明

Tested for absence of endonucleases, nicking activity, exonucleases, RNases, nucleic acids, and proteases according to the current Quality Control procedures.

其他说明

仅用于生命科学研究。不可用于诊断。

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

does not flash

闪点(°C)

does not flash


历史批次信息供参考:

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Lot/Batch Number

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With the exception of lamina-associated domains, the radial organization of chromatin in mammalian cells remains largely unexplored. Here we describe genomic loci positioning by sequencing (GPSeq), a genome-wide method for inferring distances to the nuclear lamina all along the nuclear
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Methods in molecular biology (Clifton, N.J.), 969, 43-54 (2013-01-09)
In vitro transcription of DNA with phage RNA polymerases is currently the most efficient method to produce long sequence-specific RNA. While the reaction can yield large quantities of RNA, it contains impurities due to various unwanted activities of the polymerases.
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