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T8787

Sigma-Aldrich

Triton™ X-100

Triton™ X-100

for molecular biology

别名:

4-(1,1,3,3-四甲基丁基)苯基-聚乙二醇, t-辛基苯氧基聚乙氧基乙醇, 聚乙二醇 叔辛基苯醚

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50 ML
$80.40
100 ML
$111.00
250 ML
$226.00

$80.40

预计发货时间2025年1月27日详情

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50 ML
$80.40
100 ML
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About This Item

线性分子式:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS号:
9036-19-5
Beilstein:
2315025
MDL號碼:
MFCD00128254
分類程式碼代碼:
12161900
PubChem物質ID:
329826885
NACRES:
NA.21

$80.40

预计发货时间2025年1月27日详情

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生物源

synthetic (organic)

品質等級

200

等級

for molecular biology

描述

non-ionic

形狀

liquid

技術

protein purification: suitable
protein quantification: suitable
western blot: suitable

雜質

<1.00% water (Karl Fischer)

pH值

9.7

mp

6 °C

轉變溫度

cloud point 65 °C
pour point ~7 °C

溶解度

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

密度

1.06 g/mL at 25 °C (lit.)

正離子痕跡

Fe: <5 ppm
K: <0.05%
Na: <0.1%
heavy metals: <5 ppm

應用

hematology
histology

異物活動

DNase and RNase, none detected

儲存溫度

room temp

SMILES 字串

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI 密鑰

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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相关类别

一般說明

Triton™ X-100是一种常用的非离子型的表面活性剂和乳化剂，经常用于生化应用以溶解蛋白质。它被视为一种不变性的相对温和的去污剂，并且在许多参考文献中作为一种常规被添加的试剂而被报道。它用于裂解细胞以提取蛋白和细胞器。它还可通透活细胞膜用于转染。[1][2]

應用

Triton™ X-100已用于：
  • 免疫组化用于平面视网膜的染色[3]
  • 与冰冷的PBS（磷酸盐缓冲液）一起悬浮细胞用于细胞DNA分析和Annexin V检测[4]
  • 在免疫荧光显微研究中对细胞进行通透化处理[5]
  • 在LDH检测中作为阳性对照用于测定细胞膜的完整性[6]
  • 采用改良的Belfrage和Vaughan放射性酶法过程测定肝素后血浆中的脂肪酶活性[7]
  • 外层膜蛋白提取物的制备[8]
  • 作为提取缓冲液中一种成分与tris-HCl、NaCl、CaCl2、ZnCl2、Brij 35一起用于小鼠肺细胞的匀浆[9]
  • 免疫荧光标记的组织切片处理[10]

生化／生理作用

广泛使用的非离子型表面活性剂，用于在温和的非变性条件下回收膜组分。

特點和優勢

  • 适合灵敏的分子生物学研究
  • 已检测DNA酶和RNA酶
  • 非离子型非变性去污剂
  • 优异的润湿剂和乳化剂
  • 多用表面活性剂

其他說明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)
如需了解生化试剂系列的更多信息，请填写此表。

法律資訊

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

483.8 °F - closed cup

閃點（°C）

251 °C - closed cup

個人防護裝備

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000407174
0000407173
0000407174
0000407173
0000399024

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Eric Soupene et al.
Experimental biology and medicine (Maywood, N.J.), 233(5), 507-521 (2008-04-01)
Acyl-CoA synthetase enzymes are essential for de novo lipid synthesis, fatty acid catabolism, and remodeling of membranes. Activation of fatty acids requires a two-step reaction catalyzed by these enzymes. In the first step, an acyl-AMP intermediate is formed from ATP.
M A Ferguson et al.
Journal of applied physiology (Bethesda, Md. : 1985), 85(3), 1169-1174 (1998-09-08)
The purpose of this study was to determine the threshold of exercise energy expenditure necessary to change blood lipid and lipoprotein concentrations and lipoprotein lipase activity (LPLA) in healthy, trained men. On different days, 11 men (age, 26.7 +/- 6.1
Jean-Sébastien Joyal et al.
Blood, 117(22), 6024-6035 (2011-03-01)
The failure of blood vessels to revascularize ischemic neural tissue represents a significant challenge for vascular biology. Examples include proliferative retinopathies (PRs) such as retinopathy of prematurity and proliferative diabetic retinopathy, which are the leading causes of blindness in children
Sealing the skin barrier around transcutaneous implants: in vitro study of keratinocyte proliferation and adhesion in response to surface modifications of titanium alloy.
Pendegrass C J, et al.
The Journal of Bone and Joint Surgery. British Volume, 90(1), 114-121 (2008)
Effects of four different single exercise sessions on lipids, lipoproteins, and lipoprotein lipase.
Ferguson M A, et al.
Journal of Applied Physics, 85(3), 1169-1174 (1998)
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Questions

1–6 of 6 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. What is the concentration of Triton X-1000 T8787?

    1 answer

    1. This product is considered a neat or pure liquid at 100% active ingredient. The maximum allowable water content is 1%. This value is lot specific and reported on the Certificate of Analysis.

      Helpful?

  4. Hello, I am wondering how could I find the purity of the Triton X-100 in your products. Your help and advice is appreciated. Kind regards, Soheil

    1 answer

    1. This product is 100 % active ingredient. The maximum allowable water content is 1 % for this product.

      Please see the link below to review a sample Certificate of Analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T8787-VAR0000267901.pdf

      Helpful?

  5. Can pure Triton X-100 be stored at and remain stable at less than 4C?

    1 answer

    1. This product has been subjected, in the pure form, to temperatures at or near the pour point (1 °C) with no degradation. At lower temperatures, the material may become viscous and require gentle warming to ensure homogeneity. In dilute solutions, such as cell lysates or protein samples, Triton X-100 is an excellent freeze/thaw stabilizer.

      Helpful?

  6. What form is this in?

    1 answer

    1. This product is in liquid form at room temperature (melting point is 6 deg C) as listed in the "Properties" section of the product detail page.

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