The most convenient reagent for the labeling of a peptide with DOTA [1]. This reagent has good solubility in DMF and DCM, and its free carboxyl group can be easily activated using uronium or phosphonium coupling reagents, enabling it to be attached to peptide amino groups. Removal of the t-butyl ester groups from the DOTA occurs during the course of the TFA-mediated cleavage reaction. The progress of the reaction should be monitored as the cleavage of these esters is slow, owing to the proximity of the basic ring nitrogens.
Literature references
[1] L. M. De León-Rodrýguez & Z. Kovacs (2008) Bioconjugate Chem, 19, 391.
分析说明
Colour (visual): white to grey Appearance of substance (visual): powder Identity (IR): passes test Assay (NMR): ≥ 95.0 % Identity (NMR): passes test NMR-spectrum: passes test
法律信息
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany
The spectral properties of Novabiochem®’s chromogenic and fluorogenic derivatives are invaluable tools for biochemistry, having numerous applications in enzymology, protein chemistry, immunology and histochemistry. Novabiochem® range offers one of the most extensive ranges of labeling reagents for the synthesis of such peptides, including the unique NovaTag™ resins for the production of C-terminally-labeled peptide
