推荐产品
品質等級
形狀
liquid
比活性
≥20,000 units/mL
製造商/商標名
Calbiochem®
儲存條件
OK to freeze
異物活動
Endonuclease and RNase, none detected
運輸包裝
wet ice
儲存溫度
−20°C
一般說明
Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.
Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes, and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.
生化/生理作用
Cell permeable: no
Primary Target
inactivates RNase
inactivates RNase
Product does not compete with ATP.
Reversible: no
警告
Toxicity: Standard Handling (A)
單位定義
One unit is defined as the amount of material that will inhibit 50% of the activity of 5 ng of RNase A.
外觀
In 50 mM KCl, 20 mM HEPES, 8 mM DTT, 50% glycerol.
重構
Maintain at -20°C. Avoid freezing solutions. Addition of DTT is recommended to maintain 8 mM level.
其他說明
Saxena, S.K., et al. 1992. J. Biol. Chem. 267, 21982.
Lee, F.S., et al. 1989. Biochemistry 28, 225.
Lee, F.S., et al. 1989. Biochemistry 28, 225.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
Nucleic acids research, 48(2), 847-861 (2019-12-06)
RNase E is a 472-kDa homo-tetrameric essential endoribonuclease involved in RNA processing and turnover in Escherichia coli. In its N-terminal half (NTH) is the catalytic active site, as also a substrate 5'-sensor pocket that renders enzyme activity maximal on 5'-monophosphorylated
BMC genomics, 20(1), 995-995 (2019-12-21)
Flavonoids are produced in all flowering plants in a wide range of tissues including in berry fruits. These compounds are of considerable interest for their biological activities, health benefits and potential pharmacological applications. However, transcriptomic and genomic resources for wild
The Journal of general virology, 101(6), 676-684 (2020-05-08)
Rose rosette virus (RRV) (genus Emaravirus) is the causal agent of the homonymous disease, the most destructive malady of roses in the USA. Although the importance of the disease is recognized, little sequence information and no full genomes are available
Reproduction (Cambridge, England) (2019-12-04)
We previously reported that sperm binding to cultured monolayers of bovine uterine epithelial cells induces an acute inflammatory response involving the Toll-like receptor (TLR2) signaling pathway. This response serves to clear the uterus of sperm and thereby prepares the endometrium
STAR protocols, 3(4), 101735-101735 (2022-10-02)
Here, we describe a protocol to remove single identified cells directly from Drosophila living brains and analyze their transcriptome. We detail the steps to harvest fluorescent cells using a capillary under epifluorescence and transmitted light to avoid contamination. We then
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门