T1819

Trizma^® hydrochloride solution

pH 7.0, BioPerformance Certified, 1 M, suitable for cell culture

• Synonym(s): Tris hydrochloride solution
• NACRES: NA.25
• UNSPSC Code: 12161700

Properties

• Quality Segment: 200
• grade: BioPerformance Certified, Molecular Biology
• form: liquid
• concentration: 1 M
• technique(s): cell culture | mammalian: suitable
• impurities: DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)
• pH: 7.0
• useful pH range: 7.0-9.0
• absorption: ≤0.05 at 290 at 40%

Description

General description

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Tris(hydroxymethyl)aminomethane (Tris) is a standard buffer in biological sciences and has a pH buffering range between 7.0 - 9.0. It is applicable in electrophoretic studies and capillary electrochromatography.

Application

Trizma^® hydrochloride solution has been used:

• as a buffer in reverse transcription and DNase I treatment reactions for RNA isolated from Toxoplasma gondii[1]
• in the preparation of tris- ethylenediaminetetraacetic acid (EDTA) (TE) buffer for bacterial pellet resuspension for plasmid isolation studies[2]
• for polylactic acid nanoparticle resuspension, storage, and pH-based stability studies

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type ABEK (EN14387) respirator filter