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T1819

Sigma-Aldrich

Trizma® hydrochloride solution

Trizma® hydrochloride solution
1 of 1 reviewers received a sample product or took part in a promotion

pH 7.0, BioPerformance Certified, 1 M, suitable for cell culture

Synonym(s):

Tris hydrochloride solution

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100 ML
$48.40
1 L
$90.90

$48.40

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100 ML
$48.40
1 L
$90.90

About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

$48.40

In StockDetails

Request a Bulk Order

grade

BioPerformance Certified
for molecular biology

Quality Level

200

form

liquid

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected
 bioburden, tested
 endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

7.0

useful pH range

7.0-9.0

absorption

≤0.05 at 290 at 40%

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General description

Tris(hydroxymethyl)aminomethane (Tris) is a standard buffer in biological sciences and has a pH buffering range between 7.0 - 9.0.[1] It is applicable in electrophoretic studies and capillary electrochromatography.[2]
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Application

Trizma® hydrochloride solution has been used:
  • as a buffer in reverse transcription and DNase I treatment reactions for RNA isolated from Toxoplasma gondii[3]
  • in the preparation of tris- ethylenediaminetetraacetic acid (EDTA) (TE) buffer for bacterial pellet resuspension for plasmid isolation studies[4]
  • for polylactic acid nanoparticle resuspension, storage, and pH-based stability studies[5]

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
SLCQ5982
0000367041
0000367040
0000332498
0000332500

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Production and characterisation of biodegradable PLA nanoparticles loaded with thymol to improve its antimicrobial effect
Marcet I, et al.
Journal of Food Engineering, 239 (239)
Junya Yamagish et al.
Methods in molecular biology (Clifton, N.J.), 1201, 193-205 (2014-11-13)
Parasites change their transcriptional systems in different developmental stages and in response to environmental changes. To investigate the molecular mechanisms that underlie transcriptional regulation, it is essential to identify the exact positions of the transcriptional start sites (TSSs) and characterize
A review of acellular immersion tests on bioactive glasses-influence of medium on ion release and apatite formation
Nommeots-Nomm A, et al.
International Journal of Applied Glass Science, 11(3) (11)
(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions-a review
Ferreira CMH, et al.
Royal Society of Chemistry Advances, 5(39) (5)
Kaichun Zhu et al.
Nature protocols, 1(6), 3088-3093 (2007-04-05)
This protocol describes a streamlined method of plasmid DNA extraction by continual thermal lysis, a modification of the basic boiling lysis technique, to simplify the processing of large volumes of Escherichia coli cultures. Fermented bacteria are harvested using a hollow
1 Review This action will navigate to reviews.

Questions

  1. What are the storage conditions for this product?

    1 answer

    1. This product is to be stored in room temperature. Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.

      For more safety and handling information, please view the product Safety Data Sheet:
      https://www.sigmaaldrich.com/sds/sigma/t1819

      Helpful?

Reviews

1 of 1 reviewers received a sample product or took part in a promotion

Active Filters

    • Review 1
    • Votes 0
    4 out of 5 stars.

    NaOCl Sodium Hypochlorite

    All good, however, the delivery time was longer than expected.

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