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T2569
Trizma® hydrochloride solution
pH 7.8, BioPerformance Certified, 1 M, suitable for cell culture
Synonym(s):
Tris hydrochloride solution
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100 ML
$36.80
1 L
$130.00
About This Item
UNSPSC Code:
12161700
NACRES:
NA.25
Recommended Products
grade
BioPerformance Certified
for molecular biology
Quality Level
form
solution
concentration
1 M
technique(s)
cell culture | mammalian: suitable
impurities
DNase, RNase, Protease, none detected
bioburden, tested
endotoxin, tested
≤5 ppm Heavy metals (as Pb)
pH
7.8
useful pH range
7.0-9.0
absorption
≤0.05 at 290 at 40%
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General description
Tris(hydroxymethyl)aminomethane (Tris) is a routine buffer in biological sciences. The pH buffering range is between 7.0 -9.0.[1] It displays negligible binding towards metal in solutions.[2] Tris is used majorly in combination with ethylenediaminetetraacetic acid (EDTA), especially for electrophoretic studies.[3] Its low ionic mobility finds usage in capillary electrochromatography as well.[3]
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.
Application
Trizma® hydrochloride solution has been used:
- as a component of lysis buffer for lysing human embryonic kidney (HEK) 293 cells[4]
- in the 10X digoxigenin (DIG) deoxynucleoside triphosphate (dNTP) nick translation labeling buffer and in tris EDTA and sodium chloride (TEN) buffer[5]
- in the preparation of tris, acetate, ethylenediaminetetraacetic acid (EDTA) (1x TAE) buffer and as a component of 10X gel loading dye[6]
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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