UHPLC Analysis of p-Coumaric Acid on Titan C18

column

Titan C18, 10 cm x 2.1 mm I.D., 1.9 μm particles (577124-U)

mobile phase

[A] 10 mM ammonium acetate, pH 7.0; [B] acetonitrile (70:30, A;B)

flow rate

0.3 mL/min

column temp.

35 °C

detector

MS, XIC m/z 163.15

injection

2 μL

sample

300 ng/mL each in acetonitrile:water (3:1)