LC/MS/MS Analysis of Testosterone in Human Serum on Ascentis® Express C18 after Extraction with BioSPME using C18 SPME LC Tips
Materiały
Włókno SPME
Numer produktu
Opis
Cennik
kolumna analityczna
Numer produktu
Opis
Cennik
standardowe
Numer produktu
Opis
Cennik
Testosterone solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
sample/matrix
human plasma
SPME fiber
SPME LC Tips C18 (57234-U)
extraction
60 min with 1 mL sample
desorption process
30 min with 200 μL of methanol
column
Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)
mobile phase
[A] 0.1% formic acid in water ; [B] methanol
gradient
5% B to 70% B in 3 min, then to 90% B in 0.1 min, hold 90% B for 0.9 min
flow rate
0.5 mL/min
pressure
4351 psi (300 bar)
column temp.
40 °C
detector
MS/MS, ESI(+), MRM (See Figure for transitions)
injection
10 μL
sample
10 ng/mL in methanol
Opis
Komentarz do analizy
Sensitive method for analysis of testosterone in human plasma.
Informacje prawne
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany