HPLC Analysis of Warfarin Enantiomers on Astec^® CYCLOBOND I 2000 (UV at 254 nm)

column

CYCLOBOND I 2000, 25 cm × 4.6 mm I.D., 5 μm particles (20024AST)

mobile phase

[A] acetonitrile: [B] acetic acid: [C] triethylamine; (100:0.3:0.25, A:B:C)

flow rate

1 mL/min

column temp.

23 °C

detector

UV, 254 nm

injection

5 μL