HPLC Analysis of Organic Acids on Discovery^® C18

column

Discovery C18, 5 cm × 4.6 mm I.D., 5 μm particles (504947)

mobile phase

[A] 0.1% TFA in [B] water:methanol; (60:40, A:B)

flow rate

2.0 mL/min

column temp.

20 °C

detector

UV, 254 nm

injection

10 μL