HPLC Analysis of α-Methyl-α-Phenylsuccinimide Enantiomers on Astec^® CHIROBIOTIC^® T

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] ethanol; [B] hexane; (20:80, A:B)

flow rate

3 mL/min

column temp.

23 °C

detector

UV, 210 nm

injection

5 μL

sample

5 mg/mL in methanol