HPLC Analysis of Flavonoids on Ascentis^® RP-Amide

column

Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)

mobile phase

[A] 0.085% orthophosphoric acid; [B] acetonitrile;

flow rate

1.0 mL/min

column temp.

30 °C

detector

UV, 280 nm

injection

10 μL

sample

as indicated in mobile phase A