567536-U
Discovery® HS F5 (5 µm) HPLC Columns
L × I.D. 25 cm × 4 mm, HPLC Column
Synonim(y):
Discovery Pentafluorophenyl PFP HPLC Column
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About This Item
Kod UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Polecane produkty
product name
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm
Materiały
stainless steel column
agency
suitable for USP L43
linia produktu
Discovery®
Właściwości
endcapped
producent / nazwa handlowa
Discovery®
opakowanie
1 ea of
zakres etykietowania
12% Carbon loading
Parametry
≤70 °C temp. range
400 bar pressure (5801 psi)
metody
HPLC: suitable
LC/MS: suitable
dł. × śr. wewn.
25 cm × 4 mm
powierzchnia
300 m2/g
pokrycie powierzchni
4 μmol/m2
zanieczyszczenia
<10 ppm metals
macierz
silica gel, high purity, spherical particle platform
fully porous particle
grupa aktywna macierzy
PFP (pentafluorophenyl) phase
wielkość cząstki
5 μm
wielkość porów
120 Å
operating pH range
2-8
Zastosowanie
food and beverages
metoda separacji
reversed phase
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Opis ogólny
Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
Cechy i korzyści
- Unique selectivity
- Similar retention to C18 (sometimes requires stronger mobile phase)
- Excellent peak shape
- Stable, low-bleed LC-MS separations
- Scalable separations from 3 to 10μm particle sizes
Informacje prawne
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
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Chromatographic separation of arsenic species with pentafluorophenyl column and application to rice.
Koji Baba et al.
Journal of chromatography. A, 1354, 109-116 (2014-06-21)
Arsenic species, including arsenous acid, arsenic acid, methylarsonic acid, and dimethylarsinic acid, were determined using HPLC-ICPMS. The species were separated with a Discovery HS F5 column and a simple, volatile, and isocratic mobile phase of 0.1% (v/v) formic acid and
Federica Pellati et al.
Journal of chromatography. A, 1165(1-2), 58-66 (2007-08-07)
In this study, the chromatographic performance of a pentafluorophenylpropyl (PFPP) stationary phase was evaluated for the rapid separation of phenethylamine alkaloids (i.e. (+/-)-octopamine, (+/-)-synephrine, tyramine, N-methyltyramine and hordenine) in Citrus aurantium plant material (fruits and peel), various Citrus species, extracts
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