ZRB2680

Anti-PAFAH/PLA2G7 Antibody, clone 1E9 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonim(y): 1-alkyl-2-acetylglycerophosphocholine esterase, 2-acetyl-1-alkylglycerophosphocholine esterase, EC:3.1.1.47, Group-VIIA phospholipase A2, LDL-PLA(2), LDL-associated phospholipase A2, PAF 2-acylhydrolase, PAF acetylhydrolase, Platelet-activating factor acetylhydrolase, gVIIA-PLA2
• Kod UNSPSC: 12352203
• NACRES: NA.43

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 200
• rekombinowane: expressed in HEK 293 cells
• białko sprzężone: unconjugated
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: 1E9, recombinant monoclonal
• opis: recombinant, expressed in HEK 293 cells
• linia produktu: ZooMAb^® learn more
• Postać: lyophilized
• masa cząsteczkowa: calculated mol wt 50.08 kDa; observed mol wt ~38 kDa
• oczyszczone przez: using Protein A
• reaktywność gatunkowa: rat, human, mouse
• opakowanie: antibody small pack of 25
• charakterystyka ekologicznej alternatywy: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• rozszerzona walidacja: recombinant expression; Learn more about Antibody Enhanced Validation
• metody: affinity binding assay: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• izotyp: IgG
• sekwencja epitopowa: N-terminal half
• numer dostępu Protein ID: NP_001161829
• numer dostępu UniProt: Q13093
• kategoria ekologicznej alternatywy: Aligned
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C
• informacje o genach: human ... PLA2G7(7941)

Opis

Opis ogólny

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specyficzność

Clone 1E9 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Platelet-activating factor acetylhydrolase (PAFAH). it targets an epitope within 23 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal half of human Platelet-activating factor acetylhydrolase (PAFAH).

Zastosowanie

Quality Control Testing

Evaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected PAFAH/PLA2G7 in RAW264.7 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PAFAH/PLA2G7 in lysates from HL-60 cells, Human spleen tissue and Rat spleen tissue.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected PAFAH/PLA2G7 in Human spleen tissue sections.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected PAFAH/PLA2G7 in Human spleen tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound PAFAH/PLA2G7 peptide with a KD of 1.1 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Opis wartości docelowych

Platelet-activating factor acetylhydrolase (UniProt: Q13093; also known as EC:3.1.1.47, PAF acetylhydrolase, 1-alkyl-2-acetylglycerophosphocholine esterase, 2-acetyl-1-alkylglycerophosphocholine esterase, Group-VIIA phospholipase A2, gVIIA-PLA2, LDL-associated phospholipase A2, LDL-PLA(2), PAF 2-acylhydrolase) is encoded by the PLA2G7 (also known as PAFAH) gene (Gene ID: 7941) in human. PAFAH/PLA2G7 is a lipoprotein-associated calcium-independent A2 involved in phospholipid catabolism during inflammatory and oxidative stress response. It is a secreted protein that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to produce the mature protein. In plasma, it is reported to associates with both LDL and HDL particles. At the lipid-aqueous interface it is shown to hydrolyze the ester bond of fatty acyl group attached at sn-2 position of phospholipids. It specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position, however, it can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups. PAFAH/PLA2G7 also hydrolyzes and inactivates platelet-activating factor (PAF), a potent pro-inflammatory signaling lipid that acts through PTAFR on various innate immune cells. It also hydrolyzes oxidatively truncated phospholipids carrying an aldehyde group at the omega position, preventing their accumulation in low-density lipoprotein (LDL) particles and uncontrolled pro-inflammatory effects. Mutations in PLA2G7 gene have been linked to Platelet-activating factor acetylhydrolase deficiency (PAFAD) that leads to impaired response to inflammatory agents and leads to inflammatory gastrointestinal disorders, asthma, and atopy. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Stafforini, DM., et al. (1999). J. Biol. Chem. 274(11); 7018-7024; Tjoelker, LW., et al. (1995). Nature. 374(6522); 549-553).

Postać fizyczna

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Rekonstytucja

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Przechowywanie i stabilność

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informacje prawne

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable