SAB5801021

Alpaca Anti-Mouse IgG1(Fcγ) single domain Antibody

recombinant Alpaca(VHH) Nano antibody

• Synonim(y): Recombinant Alpaca single variable domain secondary Nanobody
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: alpaca
• rekombinowane: expressed in E. coli
• białko sprzężone: unconjugated (6*his tag and one cys were added at the C terminal of the VHH)
• forma przeciwciała: affinity purified immunoglobulin
• rodzaj przeciwciała: secondary antibodies
• klon: recombinant monoclonal
• Postać: lyophilized solid
• masa cząsteczkowa: 14 kDa
• reaktywność gatunkowa: mouse, Alpaca
• metody: ELISA: 1:10000-1:50000; western blot: 1:10000-1:50000
• rozmiar: 0.1 mL
• kolor: colorless
• izotyp: VHH
• numer dostępu UniProt: P01869.2
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Alpaca Anti-Mouse IgG1(Fcγ) single domain Antibody is designed to specifically detect mouse IgG1 Fcγ fragment. It is a monovalent, single domain, recombinant antibody derived from the variable region of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgG1(Fcγ Fragment specific), Alpaca Anti-Mouse IgG1(Fcγ) single domain Antibody selectively but not with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.

Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small (less than 15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Specyficzność

Mouse IgG1(Fcγ fragment specific)
Isotype: VHH domain of alpaca IgG2b/2c

Immunogen

Recombinant Alpaca single variable domain secondary nano antibody

Opakowanie

100 μg in Glass bottle

Postać fizyczna

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Rekonstytucja

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Przechowywanie i stabilność

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C

Inne uwagi

It has weak cross reaction with mouse IgG3. No cross-reactivity with mouse IgG2b/2c, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H317,H412
• Zwroty wskazujące środki ostrożności: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Klasyfikacja zagrożeń: Aquatic Chronic 3 - Skin Sens. 1
• Kod klasy składowania: 13 - Non Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable