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Merck

SAB4700041

Sigma-Aldrich

Anti-CD3, low endotoxin antibody, Mouse monoclonal

clone OKT3, purified immunoglobulin, buffered aqueous solution

Synonim(y):

Anti-CD3 Antibody

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

mouse

białko sprzężone

unconjugated

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

OKT3, monoclonal

Postać

buffered aqueous solution

reaktywność gatunkowa

human

stężenie

1 mg/mL

metody

flow cytometry: suitable
immunohistochemistry: suitable

izotyp

IgG2a

numer dostępu NCBI

Warunki transportu

wet ice

temp. przechowywania

2-8°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... CD3(916)

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Opis ogólny

CD3 (cluster of differentiation 3) molecule is encoded by the gene mapped to human chromosome 11q23.3. The encoded protein exists in three isoforms, CD3ε, CD3γ and CD3δ and each contains an N-terminal extracellular domain, a transmembrane segment and a cytoplasmic domain. CD3 is a 20kDa glycoprotein expressed on the surface of all human T lymphocytes.
The mouse monoclonal antibody OKT3 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody, also known as Orthoclone OKT3 or Muromonab-CD3, has been extensively used as a drug for therapy of acute, glucocorticoid resistant rejection of allogenic renal, heart and liver transplants. It has also been investigated for use in treating T-cell acute lymphoblastic leukemia.

Zastosowanie

Anti-CD3, low endotoxin antibody, mouse monoclonal has been used to activate Jurkat cells and peripheral blood mononuclear cell (PBMC) to facilitate formation of T helper (Tfh) cells.
The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 1 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Działania biochem./fizjol.

T-cell receptor- CD3 (cluster of differentiation 3) complex including CD3ε, CD3γ and CD3δ play a vital role in inducing early metabolic events that lead to T cell activation. Mutation in CD3ε has been observed in T-B+ NK+ severe combined immunodeficiency (SCID) patients.

Cechy i korzyści

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Postać fizyczna

Solution in azide free phosphate buffered saline, pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/mg of the protein, as determined by the LAL test.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

nwg

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Klienci oglądali również te produkty

The T cell receptor/CD3 complex: a dynamic protein ensemble.
Clevers H, et al.
Annual Review of Immunology, 6, 629-662 (1988)
Florian Baumgart et al.
Nature methods, 13(8), 661-664 (2016-06-14)
We present a method to robustly discriminate clustered from randomly distributed molecules detected with techniques based on single-molecule localization microscopy, such as PALM and STORM. The approach is based on deliberate variation of labeling density, such as titration of fluorescent
The T cell receptor/CD3 complex: a dynamic protein ensemble.
Clevers, H, et al.
Annual Review of Immunology, 6, 629-662 (1988)
A novel pathogenic frameshift variant of CD3E gene in two T-B+ NK+ SCID patients from Turkey.
Firtina S
Immunogenetics, 69, 653-659 (2017)
Notch signaling induces cytoplasmic CD3 epsilon expression in human differentiating NK cells.
De Smedt M
Blood, 110, 2696-2703 (2007)

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