SAB4200714

Anti-Heat Shock Protein 70 (HSP70) antibody, Mouse monoclonal

clone BRM-22, purified from hybridoma cell culture

• Synonim(y): Anti-HSP70, Anti-HSPA1A
• Kod UNSPSC: 12352203
• NACRES: NA.41
• klon: BRM-22, monoclonal
• application: DB; ELISA; IF; IHC
• reaktywność gatunkowa: plant, bovine, rabbit, chicken, human, fish, insect, monkey, dog, rat, sea urchin, mouse
• metody: ELISA: suitable; dot blot: suitable; immunoblotting: 0.25-0.5 μg/mL using HeLa cell line extract; immunofluorescence: suitable; immunohistochemistry: suitable
• citations: 14

Właściwości

• forma przeciwciała: purified from hybridoma cell culture
• Poziom jakości: 200
• rodzaj przeciwciała: primary antibodies
• klon: BRM-22, monoclonal
• Formularz: buffered aqueous solution
• masa cząsteczkowa: antigen ~70 kDa
• reaktywność gatunkowa: plant, bovine, rabbit, chicken, human, fish, insect, monkey, dog, rat, sea urchin, mouse
• stężenie: ~1 mg/mL
• metody: ELISA: suitable; dot blot: suitable; immunoblotting: 0.25-0.5 μg/mL using HeLa cell line extract; immunofluorescence: suitable; immunohistochemistry: suitable
• izotyp: IgG1
• numer dostępu UniProt: P0DMV8
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... HSPA1A(3303) ; mouse ... HSPA1A(193740) ; rat ... HSPA1A(24472)

Opis

Opis ogólny

Anti-heat shock protein 70 (HSP70) antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the BRM-22 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with purified bovine brain HSP70. Monoclonal Anti-heat shock protein 70 (HSP70) specifically recognizes HSP70 from bovine, mouse, monkey, dog, human, rat, chicken,octopus, insects, plant, fish, rabbit and sea urchin origin.
HSP70 / heat shock protein family A (Hsp70) member 1A (HSPA1A), is encoded by the gene mapped to human chromosome 6p21.33. It is widely expressed in all tissues. The encoded protein is characterized with an N-terminal ATPase domain of 45kDa and a C-terminal substrate binding domain of 25kDa which is further subdivided into a β-sandwich subdomain of 15kDa and a C-terminal α-helical subdomain.

Immunogen

purified bovine brain HSP70

Działania biochem./fizjol.

The members of Hsp70 protein family are implicated in various cellular processes and are required for the response to environmental changes and survival. These proteins are essential constituents of the cellular network of molecular chaperones and folding catalysts. Hsp70 plays a vital role in several folding processes, such as the folding and assembly of newly synthesized proteins, refolding of misfolded and aggregated proteins. In addition, it also facilitates membrane translocation of organellar and secretory proteins and regulate the activity of regulatory proteins. Aberrations in the gene expression leads to skin color diversity.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Przechowywanie i stabilność

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Inne uwagi

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable