Yes, SYPRO Ruby Protein Blot Stain works with both nitrocellulose and polyvinylidene difluoride (PVDF) membranes. For more information, please access the Product Data Sheet which can be found in the DOCUMENTATION section under the 'More Documents' tab:
https://www.sigmaaldrich.com/product/sigma/s4942#product-documentation
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/369/103/s4817dat.pdf
S4942
SYPRO™ Ruby Protein Gel Stain
Synonim(y):
protein gel stain, SYPRO™ dye
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Wybierz wielkość
200 ML
1060,00 zł
1 L
3580,00 zł
5 L
11 680,00 zł
1060,00 zł
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Informacje o tej pozycji
NACRES:
NA.32
UNSPSC Code:
41105322
Przejdź do
Pomoc techniczna
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Pozwól nam pomócshelf life
≥6 mo. (when stored at room temperature and protected from light)
technique(s)
protein staining: suitable
fluorescence
λex 280,450 nm; λem 610 nm
Quality Level
Powiązane kategorie
1 of 4
Ta pozycja | |||
|---|---|---|---|
| technique(s) protein staining: suitable | technique(s) protein staining: suitable | technique(s) protein staining: suitable | technique(s) PCR: suitable |
| fluorescence λex 280,450 nm; λem 610 nm | fluorescence λex 300,470 nm; λem 570 nm | fluorescence - | fluorescence - |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| shelf life ≥6 mo. (when stored at room temperature and protected from light) | shelf life ≥6 mo. (when stored desiccated and protected from light at room temperature, 4 °C or 20 °C.) | shelf life - | shelf life - |
General description
SYPRO Ruby protein gel stain is a ready-to-use, ultrasensitive, luminescent stain for the detection of proteins separated by polyacrylamide gel electrophoresis (PAGE). This stain, designed especially for use in 2-D PAGE, has proven to be an excellent choice for 1-D PAGE and isoelectric focusing (IEF) gels as well. SYPRO Ruby protein gel stain attains sensitivity comparable to many silver stain techniques. However, unlike silver staining, the SYPRO Ruby gel stain:
- uses a simple staining protocol, with no possibility of overstaining
- delivers a linear quantitation range of over three orders of magnitude
- shows less protein-to-protein variability
- stains glycoproteins, lipoproteins, calcium-binding proteins, fibrillar proteins, and other difficult-to-stain proteins
- will not stain extraneous nucleic acids
- does not interfere with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry
Application
SYPRO ruby protein gel stain has been used for staining of the proteins after sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE).[1]
Legal Information
SYPRO is a trademark of Thermo-Fisher Scientific, Inc.
Disclaimer
Protect from light.
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Klasa składowania
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Chronic hypoxia alters mitochondrial composition in human macrophages.
Fuhrmann DC, et al.
Biochimica et Biophysica Acta, 1834, 2750-2760 (2013)
Mario Navarrete et al.
Clinical proteomics, 10(1), 17-17 (2013-11-19)
Serine hydrolases constitute a large enzyme family involved in a diversity of proteolytic and metabolic processes which are essential for many aspects of normal physiology. The roles of serine hydrolases in renal function are largely unknown and monitoring their activity
Christoph Schröder et al.
Methods in molecular biology (Clifton, N.J.), 785, 203-221 (2011-09-09)
Antibody microarrays are a multiplexing technique for the analyses of hundreds of different analytes in parallel from small sample volumes of few microlitres only. With sensitivities in the picomolar to femtomolar range, they are gaining importance in proteomic analyses. These
Jun Zou et al.
eLife, 4, e09406-e09406 (2015-10-17)
Truncating mutations in the giant sarcomeric protein Titin result in dilated cardiomyopathy and skeletal myopathy. The most severely affected dilated cardiomyopathy patients harbor Titin truncations in the C-terminal two-thirds of the protein, suggesting that mutation position might influence disease mechanism.
Silicatein-mediated incorporation of titanium into spicules from the demosponge Suberites domuncula.
Filipe Natalio et al.
Cell and tissue research, 339(2), 429-436 (2009-12-17)
Primmorphs (a three-dimensional sponge primary cell culture system) have been revealed to be a cell/tissue nano-factory for the production of tailor-made hybrid nanostructures. Growth of primmorphs is stimulated by the presence of a titanium alkoxide precursor tolerating titania (TiO2) concentrations
Produkty
Identyfikacja przyczyn i środków zaradczych dla wyzwań związanych z przygotowaniem próbki SDS-PAGE i optymalizacja warunków elektroforezy.
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
Protokoły
Protokół przygotowania próbki do lizy komórek i wydajnej ekstrakcji białek z hodowanych tkanek i komórek do późniejszego Western blottingu.
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
Powiązane treści
Datasheet
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Salve Sybro ruby è utilizzabile per colorare membrane blot PVDF?
1 answer-
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