R129

Monoclonal Anti-Ryanodine Receptor antibody produced in mouse

clone 34C, ascites fluid

• Synonim(y): Anti-CCO, Anti-KDS, Anti-MHS, Anti-MHS1, Anti-PPP1R137, Anti-RYDR, Anti-RYR, Anti-RYR-1, Anti-SKRR
• Numer MDL: MFCD00282461
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 200
• białko sprzężone: unconjugated
• forma przeciwciała: ascites fluid
• rodzaj przeciwciała: primary antibodies
• klon: 34C, monoclonal
• masa cząsteczkowa: antigen 565 kDa (non-mammalian vertebrates, doublet at 565 kDa representing the α and β isoforms)
• reaktywność gatunkowa: human, rat, rabbit, canine, mink, bovine, frog, fish, primate, chicken, mouse, sheep
• metody: direct immunofluorescence: suitable; immunohistochemistry (frozen sections): 1:1,000; immunoprecipitation (IP): suitable; western blot: 1:5,000
• izotyp: IgG1
• numer dostępu UniProt: P21817
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... RYR1(6261) , RYR2(6262) ; mouse ... Ryr1(20190) , Ryr2(20191) ; rat ... Ryr1(114207) , Ryr2(84025)

Opis

Opis ogólny

The antibody reacts strongly with RyR-1 (expressed predominantly in skeletal muscle and areas of the brain; also known as the α isoform) and RyR-2 (expressed predominantly in the heart muscle, but also found in stomach, endothelial cells and diffuse areas of the brain; also known as the β isoform). In immunohistochemistry, the antibody stains are consistent with the endoplasmic reticulum localization of the receptor in rat hippocampus.

The ryanodine receptor (RyR) is the channel responsible for the release of Ca²⁺ from the sarcoplasmic reticulum (SR) in muscle cells and it also plays a role in Ca²⁺ regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR-1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR-2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).

Immunogen

partially purified chicken pectoral muscle ryanodine receptors (RyR)

Zastosowanie

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Mouse monoclonal clone 34C anti-Ryanodine receptor is used to tag ryanodine receptors for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of ryanodine receptor in studies of calcium flux from the sarcoplasmic reticulum of muscle cells.

Postać fizyczna

Purified IgG1 antibody containing 0.05% sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): nwg
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)