Przejdź do zawartości
Merck
Wszystkie zdjęcia(2)

Kluczowe dokumenty

P3288

Sigma-Aldrich

Phosphate buffer pH 7.2 at 25 °C

for hematology and histology staining techniques

Synonim(y):

phosphate buffer

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych

Wybierz wielkość

1 VIAL
592,00 zł
12 VIALS
759,00 zł

592,00 zł


Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

Poproś o zamówienie zbiorcze

Wybierz wielkość

Zmień widok
1 VIAL
592,00 zł
12 VIALS
759,00 zł

About This Item

Kod UNSPSC:
12352107
NACRES:
NA.47

592,00 zł


Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

Poproś o zamówienie zbiorcze

Formularz

powder

kolor

white

pH

7.10-7.30

Zastosowanie

hematology
histology

temp. przechowywania

room temp

Opis ogólny

Phosphate buffer is the most commonly used component of irrigation solution during surgeries, to preserve the living tissues. It is also used in immunochemical staining procedures. Phosphate buffer serves as an important fixative for light and electron microscopy. Its buffering capacity is pH sensitive and is preferred to be used at physiological pH (7.2-7.4).[1]

Zastosowanie

Intended for use as a buffer in various Romanowsky type staining procedures, including Wright Stain, Wright Giemsa, Giemsa, May Grunwald, Jenner and Leishman. Phosphate buffer pH 7.2 at 25 °C has been used in oocyte collection from porcine ovaries.[2]

Rekonstytucja

Each vial will prepare 3.8 L of buffer, 8.3 mM.
Ta strona może zawierać tekst przetłumaczony maszynowo.

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number

Nie widzisz odpowiedniej wersji?

Jeśli potrzebujesz konkretnej wersji, możesz wyszukać konkretny certyfikat według numeru partii lub serii.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Klienci oglądali również te produkty

Biological Electron Microscopy: Theory, Techniques, and Troubleshooting (2011)
Low concentrations of MEM vitamins during in vitro maturation of porcine oocytes improves subsequent parthenogenetic development
Naruse K, et al.
Theriogenology, 67(2), 407-412 (2007)
Thaise L Teixeira et al.
Microbial pathogenesis, 135, 103618-103618 (2019-07-17)
P21 is a protein secreted by Trypanosoma cruzi (T. cruzi). Previous studies have shown a spectrum of biological activities performed by P21 such as induction of phagocytosis, leukocyte chemotaxis and inhibition of angiogenesis. However, the activity of P21 in T.

Powiązane treści

Learn about the clinical study of blood, blood-forming organs, and blood diseases including the treatment, prevention, and stains and dyes used in hematology testing.

Dowiedz się więcej na temat badań klinicznych krwi, narządów krwiotwórczych i chorób krwi, w tym leczenia, profilaktyki oraz barwników stosowanych w badaniach hematologicznych.

Questions

1–4 of 4 Questions  
  1. could you provide me with the Wright-Giemsa protocol referenced in the question above about how to solubilize Product P3288, Phosphate Buffer pH 7.2. We need official instructions for use and the product did not come with it.

    1 answer
    1. If this product is ordered separately, no instructions are provided. Instructions for preparing P3288 are found in the WG Instructions for Use that ship with each bottle of WG16, WG32, WG80, or WG128. Below is the information from the Reagent section of the WG procedure Instructions For Use

      The phosphate buffer should be prepared by diluting 1 vial of buffer to 1 gallon or 3.8 liters of deionized water. Mix well to dissolve. Discard the working phosphate solution if turbidity or visible bacterial growth is present. Store the working phosphate buffer solution at 2-8°C

      Helpful?

  2. Once made how long is the solution good for?

    1 answer
    1. The long term stability of this product in solution has not been evaluated. Upon reconstitution, the solution should be observed for any particulate matter or turbidity which would indicate possible microbial growth. However, similar prepared solutions such as P3619, a 1M Phosphate Buffer, are not assigned an expiration date. These products are quite stable and offer a warranty of 1 year from the date of shipment.

      Helpful?

  3. How to solubilize Product P3288, Phosphate Buffer pH 7.2?

    1 answer
    1. The instructions for reconstitution of Product P3288, Phosphate buffer pH 7.2 at 25 °C are indicated on the Wright-Giemsa protocol (see attached packaging insert).Briefly, Product P3288, Phosphate buffer pH 7.2 should be prepared by diluting 1 vial of buffer to 1 gallon or 3.8 liters of deionized water. Mix well to dissolve. Store Phosphate Buffer at room temperature (18-26°C). Store working phosphate buffer solution at 2-8°C. Discard the working phosphate solution if turbidity or visible bacterial growth is present.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

Reviews

No rating value

Active Filters

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej