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NA1111-1KT
745,00 zł
745,00 zł
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Informacje o tej pozycji
NACRES:
NA.52
UNSPSC Code:
41105501
Przejdź do
Pomoc techniczna
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Pozwól nam pomócusage
sufficient for 70 purifications
technique(s)
DNA purification: suitable
input
sample(s) (gel slices)
test parameters
sample volume: 3.5 g agarose
storage temp.
15-25°C
Powiązane kategorie
1 of 4
Ta pozycja | |||
|---|---|---|---|
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA purification: suitable |
| usage sufficient for 70 purifications | usage sufficient for 70 purifications | usage sufficient for 350 purifications | usage sufficient for 15 purifications |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
The GenElute Gel Extraction Kit is designed for the rapid purification of linear and plasmid DNA fragments from standard or low-melting agarose gels. This kit can also be used to purify DNA from polyacrylamide gels. Typical recovery of DNA is up to 80%. Each column can bind up to 10 μg of DNA from up to a 3.5 g agarose slice.
Application
The isolated DNA is suitable for a variety of downstream applications, such as sequencing, PCR, restriction digestion, cloning and labeling.
GenElute™ Gel Extraction Kit has been used for the purification of DNA fragments from agarose gels.
Biochem/physiol Actions
The GenElute Gel Extraction Kit combines silica-binding technology with the convenience of a spin or vacuum column format. DNA fragments of interest are extracted from slices of an agarose gel and are bound to a silica membrane. Contaminants are removed by a simple spin or vacuum wash. The bound DNA is then eluted.
The purified DNA is suitable for a variety of downstream applications, such as automated DNA sequencing, PCR, restriction digestion, cloning, and labeling.
The purified DNA is suitable for a variety of downstream applications, such as automated DNA sequencing, PCR, restriction digestion, cloning, and labeling.
The GenElute Gel Extraction Kit combines silica-binding technology with the convenience of a spin or vacuum column format. DNA fragments of interest are extracted by solubilizing slices of an agarose gel. The Gel Solubilization Solution dissolves an agarose gel slice from gels run in TBE or TAE buffer. The extracted DNA fragments selectively adsorb onto the silica membrane in the presence of the Gel Solubilization Solution. Contaminants are removed by a simple spin or vacuum wash. Finally, the bound DNA is eluted in Tris buffer.
Features and Benefits
- Bind up to 10 μg of DNA
- Recoveries up to 80%
- Up to 3.5 g can be processed per column
- Compatible with both standard and low-melting agarose in TAE or TBE buffer
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Ta strona może zawierać tekst przetłumaczony maszynowo.
Tylko elementy zestawu
Numer produktu
Opis
- Gel Solubilization Solution
- Elution Solution (10 mM Tris-HCl, pH 9.0)
Elementy zestawu są też dostępne oddzielnie
Numer produktu
Opis
Karta charakterystyki
- C2112Column Preparation Solution
related product
Numer produktu
Opis
Cennik
signalword
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C - STOT SE 3
target_organs
Respiratory system
supp_hazards
Klasa składowania
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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Protokoły
The GenElute Gel Extraction kit is designed for the rapid purification of 50 bp to 10 kb linear DNA fragments and plasmids from standard or low-melting agarose gels.
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