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NA1111

Sigma-Aldrich

GenElute Gel Extraction Kit

sufficient for 70 purifications

Synonim(y):

DNA gel extraction kit, DNA gel isolation kit, DNA gel purification kit, PCR gel clean up kit, PCR gel isolation kit, gel isolation kit, gel purification kit, Rapid purification of linear DNA

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1 KIT
717,00 zł

717,00 zł


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1 KIT
717,00 zł

About This Item

Kod UNSPSC:
41105501
NACRES:
NA.52

717,00 zł


Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

zastosowanie

sufficient for 70 purifications

Poziom jakości

metody

DNA purification: suitable

temp. przechowywania

15-25°C

Opis ogólny

The GenElute Gel Extraction Kit is designed for the rapid purification of linear and plasmid DNA fragments from standard or low-melting agarose gels. This kit can also be used to purify DNA from polyacrylamide gels. Typical recovery of DNA is up to 80%. Each column can bind up to 10 μg of DNA from up to a 3.5 g agarose slice.

Zastosowanie

GenElute Gel Extraction Kit has been used for the purification of DNA fragments from agarose gels.[1][2][3]
The isolated DNA is suitable for a variety of downstream applications, such as sequencing, PCR, restriction digestion, cloning and labeling.

Cechy i korzyści

  • Bind up to 10 μg of DNA
  • Recoveries up to 80%
  • Up to 3.5 g can be processed per column
  • Compatible with both standard and low-melting agarose in TAE or TBE buffer

Zasada

The GenElute Gel Extraction Kit combines silica-binding technology with the convenience of a spin or vacuum column format. DNA fragments of interest are extracted from slices of an agarose gel and are bound to a silica membrane. Contaminants are removed by a simple spin or vacuum wash. The bound DNA is then eluted.

The purified DNA is suitable for a variety of downstream applications, such as automated DNA sequencing, PCR, restriction digestion, cloning, and labeling.
The GenElute Gel Extraction Kit combines silica-binding technology with the convenience of a spin or vacuum column format. DNA fragments of interest are extracted by solubilizing slices of an agarose gel. The Gel Solubilization Solution dissolves an agarose gel slice from gels run in TBE or TAE buffer. The extracted DNA fragments selectively adsorb onto the silica membrane in the presence of the Gel Solubilization Solution. Contaminants are removed by a simple spin or vacuum wash. Finally, the bound DNA is eluted in Tris buffer.

Informacje prawne

GenElute is a trademark of Sigma-Aldrich Co. LLC
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Cennik

Piktogramy

CorrosionExclamation mark

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Klasyfikacja zagrożeń

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C - STOT SE 3

Organy docelowe

Respiratory system

Zagrożenia dodatkowe

Kod klasy składowania

8A - Combustible corrosive hazardous materials

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Protokoły

The GenElute Gel Extraction kit is designed for the rapid purification of 50 bp to 10 kb linear DNA fragments and plasmids from standard or low-melting agarose gels.

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