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PLN70

Sigma-Aldrich

GenElute™ Plasmid Miniprep Kit

greener alternative

sufficient for 70 purifications

Synonim(y):

GenElute™ Plasmid Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute

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About This Item

Kod UNSPSC:
41105501
NACRES:
NA.52

zastosowanie

sufficient for 70 purifications

Poziom jakości

200

charakterystyka ekologicznej alternatywy

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

metody

DNA purification: suitable

kategoria ekologicznej alternatywy

, Aligned

temp. przechowywania

15-25°C

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Opis ogólny

The GenElute Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 15 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Zastosowanie

The DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transformation, transcription, conventional, and automated sequencing.

Cechy i korzyści

  • Purified plasmid DNA in less than 30 minutes
  • Purify up to 15 μg of plasmid DNA
  • No detectable genomic DNA or RNA contamination
  • No phenol/chloroform extraction or alcohol precipitation required

Inne uwagi

For additional information, please see www.sigma-aldrich.com/genelutehp.

Informacje prawne

GenElute is a trademark of Sigma-Aldrich Co. LLC
This page may contain text that has been machine translated.

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H226,H290,H302,H315,H319,H334,H336

Klasyfikacja zagrożeń

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organy docelowe

Central nervous system

Kod klasy składowania

3 - Flammable liquids

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Resuspension Solution Add RNase A before use. After addition of RNAse, store at 2-8ºC. )

Sigma-Aldrich

R1149

Resuspension Solution

GenElute™ Plasmid Maxiprep Kit sufficient for 15 purifications

Sigma-Aldrich

PLX15

GenElute™ Plasmid Maxiprep Kit

Wash Solution 2

Sigma-Aldrich

W4639

Wash Solution 2

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Protokoły

Escort™ III Transfection Reagent Protocol

The product bulletin providin detailed use protocol for easy DNA transfection.

Protokół odczynnika do transfekcji Escort™ III

Biuletyn produktu zawiera szczegółowy protokół użytkowania ułatwiający transfekcję DNA.

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

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